Rabbit anti-Human GPR77 Polyclonal Antibody | anti-C5AR2 antibody

GPR77, CT (C5a Anaphylatoxin Chemotactic Receptor C5L2, G-protein Coupled Receptor 77, C5L2)

Cat. Num. AAA649343

• Gene Names: C5AR2; C5L2; GPF77; GPR77
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: GPR77; Polyclonal Antibody; CT (C5a Anaphylatoxin Chemotactic Receptor C5L2; G-protein Coupled Receptor 77; C5L2); Anti -GPR77; anti-C5AR2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human GPR77.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% sodium azide.

• Applicable Applications for anti-C5AR2 antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Synthetic peptide corresponding to 20aa from C-terminal cytoplasmic domain of human GPR77.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with GPR77 antibody at 10ug/ml followed by biotinylated goat rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-C5AR2 antibody

GPR77 is an Anaphylatoxin Receptor. Binding to anaphylatoxins can result in increased triglyceride synthesis, although its precise role in cellular responses has not been determined.

Product Categories/Family for anti-C5AR2 antibody

Antibodies; Abs to G Protein Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 13122467
• NCBI GeneID: 27202
• UniProt Accession #: Q9P296; B2RA09
• Molecular Weight: 36,080 Da
• NCBI Official Full Name: G protein-coupled receptor
• NCBI Official Synonym Full Names: complement component 5a receptor 2
• NCBI Official Symbol: C5AR2
• NCBI Official Synonym Symbols: C5L2; GPF77; GPR77
• NCBI Protein Information: C5a anaphylatoxin chemotactic receptor 2; G protein-coupled receptor 77; G protein-coupled receptor C5L2; C5a anaphylatoxin chemotactic receptor C5L2
• UniProt Protein Name: C5a anaphylatoxin chemotactic receptor 2
• Protein Family: C5a anaphylatoxin chemotactic receptor
• UniProt Gene Name: C5AR2
• UniProt Synonym Gene Names: C5L2; GPR77
• UniProt Entry Name: C5AR2_HUMAN

NCBI Description

This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

Uniprot Description

Function: Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways. Ref.8 Ref.10 Ref.11

Subunit structure: Interacts with C3 (the anaphylatoxin peptide C3a and the adipogenic hormone ASP); the interaction occurs with higher affinity for ASP, enhancing the phosphorylation and activation of GPR77, recruitment of ARRB2 to the cell surface and endocytosis of GRP77. Ref.9

Subcellular location: Cell membrane; Multi-pass membrane protein Ref.11.

Tissue specificity: Frontal cortex, hippocampus, hypothalamus, pons and liver. Ref.1 Ref.2

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.