Rabbit anti-Human, Mouse PIP5K2A Polyclonal Antibody | anti-PIP5K2A antibody

PIP5K2A, NT (Phosphatidylinositol-5-phosphate 4-kinase Type-2 alpha, 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha, Diphosphoinositide Kinase 2-alpha, PIP5KIII, Phosphatidylinositol-5-phosphate 4-kinase Type II alpha, PI(5)P 4-kinase Type II alpha,

Cat. Num. AAA6493037

• Gene Names: PIP4K2A; PIPK; PI5P4KA; PIP5K2A; PIP5KIIA; PIP5KII-alpha
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PIP5K2A; Polyclonal Antibody; NT (Phosphatidylinositol-5-phosphate 4-kinase Type-2 alpha; 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha; Diphosphoinositide Kinase 2-alpha; PIP5KIII; Phosphatidylinositol-5-phosphate 4-kinase Type II alpha; PI(5)P 4-kinase Type II alpha; ; anti-PIP5K2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PIP5K2A. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PIP5K2A antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PIP5K2A (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-PIP5K2A Pab is used in Western blot to detect PIP5K2A in mouse skeletal muscle tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PIP5K2A antibody

Antibodies; Protein Kinases

References

1. Rozenvayn, N., et al., J. Biol. Chem. 278(10):8126-8134 (2003). 2. Boronenkov, I.V., et al., J. Biol. Chem. 270(7):2881-2884 (1995). 3. Loijens, J.C., et al., Adv. Enzyme Regul. 36, 115-140 (1996). 4. Divecha, N., et al., Biochem. J. 309(Pt 3), 715-719 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 6857820
• NCBI GeneID: 5305
• NCBI Accession #: NP_005019.2
• NCBI GenBank Nucleotide #: NM_005028.4
• UniProt Accession #: P48426; P53807; Q5VUX3; B0YJ66; B4DGX2; D3DRV1
• Molecular Weight: 39,802 Da
• NCBI Official Full Name: phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
• NCBI Official Synonym Full Names: phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
• NCBI Official Symbol: PIP4K2A
• NCBI Official Synonym Symbols: PIPK; PI5P4KA; PIP5K2A; PIP5KIIA; PIP5KII-alpha
• NCBI Protein Information: phosphatidylinositol 5-phosphate 4-kinase type-2 alpha; PIP5KIII; PIP5KIIalpha; PIP4KII-alpha; PI(5)P 4-kinase type II alpha; PtdIns(4)P-5-kinase B isoform; ptdIns(4)P-5-kinase C isoform; diphosphoinositide kinase 2-alpha; ptdIns(5)P-4-kinase isoform 2-al
• UniProt Protein Name: Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
• UniProt Gene Name: PIP4K2A
• UniProt Synonym Gene Names: PIP5K2; PIP5K2A; PI(5)P 4-kinase type II alpha; PIP4KII-alpha
• UniProt Entry Name: PI42A_HUMAN

NCBI Description

Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq, Jul 2008]

Uniprot Description

PIP4K2A: Catalyzes the phosphorylation of phosphatidylinositol 5- phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin- stimulated glucose uptake by lowering the levels of PtdIns5P. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.

Protein type: EC 2.7.1.149; Motility/polarity/chemotaxis; Kinase, lipid; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 10p12.2

Cellular Component: plasma membrane; cytosol; nucleus

Molecular Function: 1-phosphatidylinositol-4-phosphate 5-kinase activity; 1-phosphatidylinositol-5-phosphate 4-kinase activity; ATP binding

Biological Process: phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process