Goat Ferritin Heavy Chain / FTH1 Polyclonal Antibody | anti-FTH1 antibody

Goat Polyclonal to Human Ferritin Heavy Chain / FTH1

Cat. Num. AAA242639

• Gene Names: FTH1; FHC; FTH; HFE5; PLIF; FTHL6; PIG15
• Reactivity: Gibbon, Gorilla, Human, Monkey, Orangutan; Predicted Reactivity: Dog, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: Ferritin Heavy Chain / FTH1; Polyclonal Antibody; Goat Polyclonal to Human Ferritin Heavy Chain / FTH1; Anti-Ferritin Heavy Chain / FTH1 Antibody (C-Terminus) IHC-plus; FTH1; Apoferritin; Ferritin H subunit; Ferritin heavy chain; Ferritin; heavy polypeptide 1; FHC; FTHL6; PIG15; PLIF; FTH; Human Ferritin Heavy Chain; anti-FTH1 antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human, Monkey, Orangutan; Predicted Reactivity: Dog, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human FTH1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 183

• Applicable Applications for anti-FTH1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:8000), IHC-P (4 ug/ml), WB (0.03 - 0.1 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-DKHTLGDSDNES from the C-terminus of human FTH1 (NP_002023.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Opossum, Platypus (100%); Dolphin, Dog, Rabbit (92%); Panda, Cat, Armadillo (83%).
• Immunogen Type: Synthetic peptide
• Immunogen: Ferritin Heavy Chain / FTH1 antibody was raised against synthetic peptide C-DKHTLGDSDNES from the C-terminus of human FTH1 (NP_002023.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Opossum, Platypus (100%); Dolphin, Dog, Rabbit (92%); Panda, Cat, Armadillo (83%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-FTH1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.)

Immunohistochemistry (IHC)

(Anti-FTH1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.)

Western Blot (WB)

(Antibody (0.05 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 56682959
• NCBI GeneID: 2495
• NCBI Accession #: NP_002023.2
• NCBI GenBank Nucleotide #: NM_002032.2
• UniProt Accession #: P02794; Q3KRA8; Q3SWW1; B3KNR5
• Molecular Weight: 21,226 Da
• NCBI Official Full Name: ferritin heavy chain
• NCBI Official Synonym Full Names: ferritin, heavy polypeptide 1
• NCBI Official Symbol: FTH1
• NCBI Official Synonym Symbols: FHC; FTH; HFE5; PLIF; FTHL6; PIG15
• NCBI Protein Information: ferritin heavy chain; apoferritin; ferritin H subunit; placenta immunoregulatory factor; proliferation-inducing protein 15; cell proliferation-inducing gene 15 protein
• UniProt Protein Name: Ferritin heavy chain
• Protein Family: Iron transporter
• UniProt Gene Name: FTH1
• UniProt Synonym Gene Names: FTH; FTHL6; Ferritin H subunit
• UniProt Entry Name: FRIH_HUMAN

NCBI Description

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Ferritin-H: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Belongs to the ferritin family.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.16.3.1; Oxidoreductase

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: ferritin complex; mitochondrion; cytosol; nucleus

Molecular Function: ferroxidase activity; protein binding; ferric iron binding; iron ion binding

Biological Process: receptor-mediated endocytosis; negative regulation of cell proliferation; negative regulation of fibroblast proliferation; cellular iron ion homeostasis; immune response; post-Golgi vesicle-mediated transport; iron ion transport; transmembrane transport; intracellular sequestering of iron ion

Disease: Hemochromatosis, Type 5