Rabbit PA28, alpha Polyclonal Antibody
PA28, alpha, CT (Proteasome Activator 28-alpha subunit) (AP)
Reactivity
Human, Rhesus Macaque, Mouse, Porcine, Rat
Applications
Western Blot
Purity
Epitope-affinity purified from rabbit antiserum.
Synonyms
PA28; alpha; Polyclonal Antibody; CT (Proteasome Activator 28-alpha subunit) (AP); anti-PA28; alpha antibody
Host
Rabbit
Reactivity
Human, Rhesus Macaque, Mouse, Porcine, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Reacts with the C-terminal region of mouse, rat, human, crab-eating macaque (monkey) and pig PA28a. On Western blots, it identifies the target band at ~28kD. Faint bands of unknown origin may be observed in some homogenate or lysate preparations. Reactivity has been confirmed with mouse spleen, liver and lung homogenates, as well as with human BC-1 B-lymphocyte lymphoma lysates. Based on amino acid sequence homology, cross-reactivity with rat is expected.
Purity/Purification
Epitope-affinity purified from rabbit antiserum.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-PA28, alpha antibody
Western Blot (WB)
Application Notes
WB: 1-3ug/ml
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide derived from the C-terminal region of the proteasome activator 28-alpha subunit (PA28a, also known as 11s regulator complex alpha subunit (REG-alpha) (11S REG) and Interferon gamma up-regulated I-5111 protein (IGUP I-5111) protein.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-PA28, alpha antibody
Reactivity has been confirmed with mouse spleen, liver and lung homogenates, as well as with human BC-1 B-lymphocyte lymphoma lysates. Based on amino acid sequence homology, cross-reactivity with rat is expected.
Product Categories/Family for anti-PA28, alpha antibody
References
1. Chu-Ping M, et al. Biochem Biophys Acta 1119:303-311, 1992. 2. Jiang H, et al. Immunogenetics 46:93-98, 1997. 3. Soza A, et al. FEBS Lett 413:27-34, 1997. 4. Faries PL, et al. Ann Vasc Surg 15:628-633, 2001. 5. Wilk S, et al. Arch Biochem Biophys 384:174-80, 2000. 6. Murata S, et al. EMBO J 20:5898-907, 2001. 7. Chu-Ping M, et al. Biol Chem 268:22514-22519, 1993. 8. Fabunmi RP, et al. J Cell Sci 114:29-36, 2001. 9. Groettrup M, et al. Nature 381:166-168, 1996. 10. Kohda K, et al. J Immunol 160:4923-14935, 1998. 11. Schwarz K, et al. Eur J Immunol 30:3672-3679, 2000. 12. Matsushita M, et al. J Rheumatol 31:252-259, 2004. 13. Miyagi T, et al. J Gastroenterol Hepatol 18:32-40, 2003.
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Product Notes
The PA28, alpha (Catalog #AAA6491636) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PA28, alpha, CT (Proteasome Activator 28-alpha subunit) (AP) reacts with Human, Rhesus Macaque, Mouse, Porcine, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PA28, alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1-3ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PA28, alpha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PA28, alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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