Western Blot (WB)
(Western Blot analysis of DCTN2 expression in transfected 293T cell line by DCTN2 polyclonal antibody. Lane 1: DCTN2 transfected lysate (44.2kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human DCTN2 Polyclonal Antibody | anti-DCTN2 antibody
DCTN2 (Dynactin Subunit 2, Dynactin Complex 50kD Subunit, DCTN-50, 50kD Dynein-associated Polypeptide, p50 Dynamitin, DCTN50)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot analysis of DCTN2 expression in transfected 293T cell line by DCTN2 polyclonal antibody. Lane 1: DCTN2 transfected lysate (44.2kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of DCTN2 expression in transfected 293T cell line by DCTN2 polyclonal antibody. Lane 1: DCTN2 transfected lysate (44.2kD). Lane 2: Non-transfected lysate.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012]
Uniprot Description
Function: Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.
Subunit structure: Subunit of dynactin, a multiprotein complex associated with dynein. Interacts with BICD2 and CEP135
By similarity. Interacts with DYNAP, ECM29 and MAPRE1. Ref.9 Ref.13 Ref.14
Subcellular location: Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Membrane; Peripheral membrane protein Ref.1.
Sequence similarities: Belongs to the dynactin subunit 2 family.
Sequence caution: The sequence AAO34395.1 differs from that shown. Reason: Frameshift at position 381.
Research Articles on DCTN2
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Product Notes
The DCTN2 dctn2 (Catalog #AAA649021) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DCTN2 (Dynactin Subunit 2, Dynactin Complex 50kD Subunit, DCTN-50, 50kD Dynein-associated Polypeptide, p50 Dynamitin, DCTN50) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DCTN2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the DCTN2 dctn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MADPKYADLP GIARNEPDVY ETSDLPEDDQ AEFDAEELTS TSVEHIIVNP NAAYDKFKDK RVGTKGLDFS DRIGKTKRTG YESGEYEMLG EGLGVKETPQ QKYQRLLHEV QELTTEVEKI KTTVKESATE EKLTPVLLAK QLAALKQQLV ASHLEKLLGP DAAINLTDPD GALAKRLLLQ LEATKNSKGG SGGKTTGTPP DSSLVTYELH SRPEQDKFSQ AAKVAELEKR LTELETAVRC DQDAQNPLSA GLQGACLMET VELLQAKVSA LDLAVLDQVE ARLQSVLGKV NEIAKHKASV EDADTQSKVH QLYETIQRWS PIASTLPELV QRLVTIKQLH EQAMQFGQLL THLDTTQQMI ANSLKDNTTL LTQVQTTMRE NLATVEGNFA SIDERMKKLG K. It is sometimes possible for the material contained within the vial of "DCTN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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