Mouse anti-Human WRAP53 Polyclonal Antibody | anti-WRAP53 antibody

WRAP53 (WD40 Repeat-containing Protein Encoding RNA Antisense to p53, Telomerase Cajal Body Protein 1, TCAB1, WD Repeat-containing Protein 79, WDR79, DKCB3)

Cat. Num. AAA648964

• Gene Names: WRAP53; DKCB3; TCAB1; WDR79
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: WRAP53; Polyclonal Antibody; WRAP53 (WD40 Repeat-containing Protein Encoding RNA Antisense to p53; Telomerase Cajal Body Protein 1; TCAB1; WD Repeat-containing Protein 79; WDR79; DKCB3); Anti -WRAP53 (WD40 Repeat-containing Protein Encoding RNA Antisense to p53; anti-WRAP53 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human WDR79.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MKTLETQPLAPDCCPSDQDPAPAHPSPHASPMNKNADSELMPPPPERGDPPRLSPDPVAGSAVSQELREGDPVSLSTPLETEFGSPSELSPRIEEQELSENTSLPAEEANGSLSEEEANGPELGSGKAMEDTSGEPAAEDEGDTAWNYSFSQLPRFLSGSWSEFSTQPENFLKGCKWAPDGSCILTNSADNILRIYNLPPELYHEGEQVEYAEMVPVLRMVEGDTIYDYCWYSLMSSAQPDTSYVASSSRENPIHIWDAFTGELRASFRAYNHLDELTAAHSLCFSPDGSQLFCGFNRTVRVFSTARPGRDCEVRATFAKKQGQSGIISCIAFSPAQPLYACGSYGRSLGLYAWDDGSPLALLGGHQGGITHLCFHPDGNRFFSGARKDAELLCWDLRQSGYPLWSLGREVTTNQRIYFDLDPTGQFLVSGSTSGAVSVWDTDGPGNDGKPEPVLSFLPQKDCTNGVSLHPSLPLLATASGQRVFPEPTESGDEGEELGLPLLSTRHVHLECRLQLWWCGGAPDSSIPDDHQGEKGQGGTEGGVGELI

• Applicable Applications for anti-WRAP53 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human WDR79, aa1-548 (AAH02336.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of WDR79 expression in transfected 293T cell line by WDR79 polyclonal antibody. Lane 1: WDR79 transfected lysate (60.28kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-WRAP53 antibody

Antibodies; Abs to WD Repeat Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 59726941
• NCBI GeneID: 55135
• UniProt Accession #: Q9BUR4; Q08ET9; Q9NW09; B3KPR9; D3DTQ4
• Molecular Weight: 59,309 Da
• NCBI Official Full Name: WRAP53
• NCBI Official Synonym Full Names: WD repeat containing, antisense to TP53
• NCBI Official Symbol: WRAP53
• NCBI Official Synonym Symbols: DKCB3; TCAB1; WDR79
• NCBI Protein Information: telomerase Cajal body protein 1; WD40 protein Wrap53; WD repeat-containing protein 79; WD40 repeat-containing protein encoding RNA antisense to p53
• UniProt Protein Name: Telomerase Cajal body protein 1
• Protein Family: Telomerase Cajal body protein
• UniProt Gene Name: WRAP53
• UniProt Synonym Gene Names: TCAB1; WDR79
• UniProt Entry Name: WAP53_HUMAN

NCBI Description

This gene encodes an essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex required for telomere synthesis. This protein is enriched in Cajal bodies, nuclear sites of RNP processing that are important for telomerase function. It interacts with dyskerin, TERT and TERC, other components of active telomerase, and with small Cajal body RNAs (scaRNAs), which are involved in modifying splicing RNAs. This mRNA also functions as a p53 antisense transcript, that regulates endogenous p53 mRNA levels and further induction of p53 protein by targeting the 5' untranslated region of p53 mRNA. Alternatively spliced transcript variants which differ only in the 5' UTR have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

Function: Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes. In the telomerase holoenzyme complex, it controls telomerase localization to Cajal body. Required for delivery of TERC to telomeres during S phase and for telomerase activity. Binds small Cajal body RNAs (scaRNAs). The mRNA encoding this protein plays a critical role in the regulation of p53 expression at the post-transcriptional level; it is involved both in maintaining basal p53 mRNA levels and in p53 induction upon DNA damage. Ref.11 Ref.13

Subunit structure: Component of the telomerase holoenzyme complex at least composed of TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC). Ref.13

Subcellular location: Nucleus › Cajal body. Cytoplasm Ref.11 Ref.13.

Tissue specificity: Expressed in all tissues and cell lines examined. Ref.11

Involvement in disease: Dyskeratosis congenita, autosomal recessive, 3 (DKCB3) [MIM:613988]: A rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.16

Sequence similarities: Contains 6 WD repeats.