Horse Interleukin 18 (IL18) ELISA Kit | IL18 elisa kit

Horse Interleukin 18 (IL18) ELISA Kit

Cat. Num. AAA2700997

• Gene Names: IL18; IGIF; IL-18; IL-1g; IL1F4
• Reactivity: Horse
• Synonyms: Interleukin 18 (IL18); Horse Interleukin 18 (IL18) ELISA Kit; IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma; IL18 elisa kit

• Reactivity: Horse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 18 (IL18).; No significant cross-reactivity or interference between Interleukin 18 (IL18) and analogues was observed.
• Sequence Length: 193

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 6.1pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 18 (IL18) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 18 (IL18) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL18 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 18 (IL18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 18 (IL18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 18 (IL18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 18 (IL18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504653
• NCBI GeneID: 3606
• NCBI Accession #: NP_001553.1
• NCBI GenBank Nucleotide #: NM_001562.3
• UniProt Accession #: Q14116; O75599; Q6FGY3; Q6WWJ7
• Molecular Weight: 21,896 Da
• NCBI Official Full Name: interleukin-18 isoform 1
• NCBI Official Synonym Full Names: interleukin 18
• NCBI Official Symbol: IL18
• NCBI Official Synonym Symbols: IGIF; IL-18; IL-1g; IL1F4
• NCBI Protein Information: interleukin-18
• UniProt Protein Name: Interleukin-18
• Protein Family: Interleukin
• UniProt Gene Name: IL18
• UniProt Synonym Gene Names: IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma

NCBI Description

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Uniprot Description

Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.