Rabbit anti-Human MMP12 Polyclonal Antibody | anti-MMP12 antibody

MMP12, CT (Macrophage Metalloelastase, MME, Matrix Metalloproteinase-12, MMP-12, Macrophage Elastase, ME, hME, HME) (FITC)

Cat. Num. AAA6487065

• Gene Names: MMP12; ME; HME; MME; MMP-12
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MMP12; Polyclonal Antibody; CT (Macrophage Metalloelastase; MME; Matrix Metalloproteinase-12; MMP-12; Macrophage Elastase; ME; hME; HME) (FITC); anti-MMP12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MMP12.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MMP12 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MMP12 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. MMP12(arrow) was detected using the purified Pab;)

Western Blot (WB)

(Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse kidney tissue lysates. MMP12(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP12 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MMP12 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-MMP12 antibody

Antibodies; Matrix Metalloproteinases

References

1. Nar, H., et al., J. Mol. Biol. 312(4):743-751 (2001). 2. Lang, R., et al., J. Mol. Biol. 312(4):731-742 (2001). 3. Gronski, T.J. Jr., et al., J. Biol. Chem. 272(18):12189-12194 (1997). 4. Shapiro, S.D., et al., J. Biol. Chem. 268(32):23824-23829 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 73858572
• NCBI GeneID: 4321
• NCBI Accession #: NP_002417.2
• NCBI GenBank Nucleotide #: NM_002426.4
• UniProt Accession #: P39900; Q2M1L9; B2R9X8; B7ZLF6
• Molecular Weight: 54,002 Da
• NCBI Official Full Name: macrophage metalloelastase preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 12 (macrophage elastase)
• NCBI Official Symbol: MMP12
• NCBI Official Synonym Symbols: ME; HME; MME; MMP-12
• NCBI Protein Information: macrophage metalloelastase; matrix metalloproteinase 12 (macrophage elastase)
• UniProt Protein Name: Macrophage metalloelastase
• Protein Family: Macrophage metalloelastase
• UniProt Gene Name: MMP12
• UniProt Synonym Gene Names: HME; MME; ME; hME; MMP-12
• UniProt Entry Name: MMP12_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP12: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Belongs to the peptidase M10A family.

Protein type: Secreted, signal peptide; Secreted; EC 3.4.24.65; Protease

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; positive regulation of epithelial cell proliferation involved in wound healing; proteolysis; wound healing, spreading of epidermal cells