Rabbit anti-Human IDE Polyclonal Antibody | anti-IDE antibody

IDE, ID (Insulin-degrading Enzyme, Insulysin, Insulinase, Insulin Protease, Abeta-Degrading Protease) (Biotin)

Cat. Num. AAA6483558

• Gene Names: IDE; INSULYSIN
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: IDE; Polyclonal Antibody; ID (Insulin-degrading Enzyme; Insulysin; Insulinase; Insulin Protease; Abeta-Degrading Protease) (Biotin); anti-IDE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IDE.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-IDE antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 406-435 in the central region of human IDE, UniProt Accession #NP_004960; P14735. Species sequence homology: bovine, mouse, rat
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in A375 cell line lysates (35ug/lane) using I8601-02C. IDE (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with I8601-02C, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of I8601-02C for immunohistochemistry.)

Related Product Information for anti-IDE antibody

IDE belongs to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.

Product Categories/Family for anti-IDE antibody

Antibodies; Insulin

References

1. Vepsalainen, S., J. Med. Genet. 44(9), 606-608 (2007). 2. Kim, M., J. Biol. Chem. 282(11), 7825-7832 (2007). 3. Radulescu, R.T., Int. J. Oncol. 30(1), 73-80 (2007). 4. Li, Q., Cell 127(2), 305-316 (2006). 5. Razvan T. Radulescu, et al. Mol. Cancer Ther. published 13 October 2009, 10.1158/1535-7163. MCT-09-0706. (Applications: IP) (E-pub).

NCBI and Uniprot Product Information

• NCBI GI #: 155969707
• NCBI GeneID: 3416
• NCBI Accession #: NP_004960
• NCBI GenBank Nucleotide #: NM_004969
• UniProt Accession #: P14735; Q5T5N2; B2R721; B7ZAU2; D3DR35
• Molecular Weight: 54,240 Da
• NCBI Official Full Name: insulin-degrading enzyme isoform 1
• NCBI Official Synonym Full Names: insulin-degrading enzyme
• NCBI Official Symbol: IDE
• NCBI Official Synonym Symbols: INSULYSIN
• NCBI Protein Information: insulin-degrading enzyme; Abeta-degrading protease; insulin protease; insulinase
• UniProt Protein Name: Insulin-degrading enzyme
• Protein Family: IgM protease
• UniProt Gene Name: IDE
• UniProt Synonym Gene Names: Insulinase
• UniProt Entry Name: IDE_HUMAN

Uniprot Description

IDE: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. Belongs to the peptidase M16 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Nuclear receptor co-regulator; Cell surface; EC 3.4.24.56

Chromosomal Location of Human Ortholog: 10q23-q25

Cellular Component: nucleoplasm; peroxisomal matrix; extracellular space; cell surface; mitochondrion; cytoplasm; plasma membrane; peroxisome; cytosol; nucleus

Molecular Function: ubiquitin binding; protein homodimerization activity; zinc ion binding; beta-amyloid binding; metalloendopeptidase activity; ATPase activity; peptide binding; beta-endorphin binding; insulin binding; protein binding; glycoprotein binding; ATP binding; receptor binding

Biological Process: negative regulation of proteolysis; positive regulation of protein oligomerization; proteolysis involved in cellular protein catabolic process; viral reproduction; protein heterooligomerization; beta-amyloid metabolic process; insulin receptor signaling pathway; hormone catabolic process; determination of adult life span; proteolysis; protein homooligomerization; protein homotetramerization