Western Blot (WB)
(Western blot analysis of IL27 (arrow) using rabbit polyclonal IL27 Antibody (C71). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IL27 gene.)
Rabbit anti-Human, Mouse IL-27 Polyclonal Antibody | anti-IL-27 antibody
IL-27, ID (Interleukin-17D, IL-17D, Interleukin-27, IL17D, IL27, UNQ3096/PRO21175) (PE)
Gene Names
IL17D; IL-17D
Reactivity
Human, Mouse
Applications
Immunohistochemistry
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
IL-27; Polyclonal Antibody; ID (Interleukin-17D; IL-17D; Interleukin-27; IL17D; IL27; UNQ3096/PRO21175) (PE); anti-IL-27 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human L27. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Sequence Length
202
Applicable Applications for anti-IL-27 antibody
Immunohistochemistry (IHC), FLISA
Application Notes
IHC: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the center region of human L27 (KLH).
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of IL27 (arrow) using rabbit polyclonal IL27 Antibody (C71). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IL27 gene.)
Western Blot (WB)
(Western blot analysis of IL27 (arrow) using rabbit polyclonal IL27 Antibody (C71). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IL27 gene.)
Immunohistochemistry (IHC)
(IL-27 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IL-27 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(IL-27 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IL-27 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(IL-27 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(IL-27 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-IL-27 antibody
References
1. Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). 2. Starnes, T., et al., J. Immunol. 169(2):642-646 (2002).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cytokine that shares the sequence similarity with IL17. The treatment of endothelial cells with this cytokine has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF. The increased expression of IL8 induced by this cytokine was found to be NF-kappa B-dependent. [provided by RefSeq, Jul 2008]
Research Articles on IL-27
Similar Products
Product Notes
The IL-27 (Catalog #AAA6483368) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL-27, ID (Interleukin-17D, IL-17D, Interleukin-27, IL17D, IL27, UNQ3096/PRO21175) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL-27 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA. IHC: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the IL-27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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