Mouse anti-Human STXBP4 Monoclonal Antibody | anti-STXBP4 antibody

STXBP4 (Syntaxin-binding Protein 4, Syntaxin 4-interacting Protein, STX4-interacting Protein, Synip)

Cat. Num. AAA647854

• Gene Names: STXBP4; Synip
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: STXBP4; Monoclonal Antibody; STXBP4 (Syntaxin-binding Protein 4; Syntaxin 4-interacting Protein; STX4-interacting Protein; Synip); Anti -STXBP4 (Syntaxin-binding Protein 4; anti-STXBP4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2B12
• Specificity: Recognizes human STXBP4.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MIGVSFEEAKSIITRAKLSTRLESAWEIAFIRQKSDNIQPENLSCTSLIEASGEYGPQASTLSLFSSPPEILIPKTSSTPKTNNDILSSCEIKTGYNKTVQIPITSENSTVGLSNTDVASAWTENYGLQEKISLNPSVRFKAEKLEMALNYLGIQPTKEQHQALRQQVQADSKGTVSFGDFVQVARNLFCLQLDEVNVGAHEISNILDSQLLPCDSSEADEMERLKCERDDALKEVNTLKWPGQN

• Applicable Applications for anti-STXBP4 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Sandwich ELISA: The detection limit for recombinant GST tagged STXBP4 is ~0.1ng/ml as a capture antibody.
• Immunogen: Full-length recombinant protein corresponding to aa1-246 from human STXBP4 (AAH41485) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (53.06kD).)

Testing Data

(Detection limit for recombinant GST tagged STXBP4 is 0.1ng/ml as a capture antibody.)

Product Categories/Family for anti-STXBP4 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 63999048
• NCBI GeneID: 252983
• NCBI Accession #: NP_848604.3
• NCBI GenBank Nucleotide #: NM_178509.5
• UniProt Accession #: Q6ZWJ1; Q8IVZ5
• Molecular Weight: 61,662 Da
• NCBI Official Full Name: syntaxin-binding protein 4
• NCBI Official Synonym Full Names: syntaxin binding protein 4
• NCBI Official Symbol: STXBP4
• NCBI Official Synonym Symbols: Synip
• NCBI Protein Information: syntaxin-binding protein 4; STX4-interacting protein; syntaxin 4 interacting protein; syntaxin 4-interacting protein
• UniProt Protein Name: Syntaxin-binding protein 4
• Protein Family: Syntaxin-binding protein
• UniProt Gene Name: STXBP4
• UniProt Synonym Gene Names: STX4-interacting protein; Synip
• UniProt Entry Name: STXB4_HUMAN

Uniprot Description

Function: Plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. Inhibits the translocation of SLC2A4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2. Stimulation with insulin disrupts the interaction with STX4A, leading to increased levels of SLC2A4 at the plasma membrane. May also play a role in the regulation of insulin release by pancreatic beta cells after stimulation by glucose

By similarity.

Subunit structure: Interacts with STX4A

By similarity.

Subcellular location: Cytoplasm

By similarity.

Post-translational modification: Phosphorylated on Ser-99 by PKB/AKT2 after insulin treatment. Phosphorylation on Ser-99 abolishes the interaction with STX4A

By similarity.

Sequence similarities: Contains 1 PDZ (DHR) domain.Contains 1 WW domain.