Western Blot (WB)
(ILKAP Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ILKAP antibody detected the ILKAP protein (arrow).)
Rabbit anti-Human ILKAP Polyclonal Antibody | anti-ILKAP antibody
ILKAP, ID (ILKAP, Integrin-linked kinase-associated serine/threonine phosphatase 2C)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(ILKAP Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ILKAP antibody detected the ILKAP protein (arrow).)
Western Blot (WB)
(ILKAP Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ILKAP antibody detected the ILKAP protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation. Ref.1 Ref.7
Catalytic activity: A phosphoprotein + H2O = a protein + phosphate.
Cofactor: Binds 2 magnesium or manganese ions per subunit
By similarity. Ref.1
Enzyme regulation: Inhibited rather than stimulated by magnesium.
Subunit structure: Interacts with ILK. Specific association with ILK is independent of the catalytic activity of either partner. Ref.1 Ref.7
Subcellular location: Cytoplasm Ref.1.
Tissue specificity: Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues. Ref.1
Sequence similarities: Belongs to the PP2C family.Contains 1 PP2C-like domain.
Research Articles on ILKAP
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Product Notes
The ILKAP ilkap (Catalog #AAA647697) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ILKAP, ID (ILKAP, Integrin-linked kinase-associated serine/threonine phosphatase 2C) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ILKAP can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the ILKAP ilkap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ILKAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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