Immunoprecipitation (IP)
(Immunoprecipitation Analysis: Representative lot data. 10 ug of MBS630808 immunoprecipitated DARPP-32 from 500 ug of rat brain RIPA lysate. Arrow indicates DARPP-32 (~32kDa).)
Rabbit anti-Mouse, Rat DARPP32 Polyclonal Antibody | anti-DARPP32 antibody
DARPP32 (Dopamine and cAMP-regulated Phosphoprotein DARPP32, DARPP 32, DARPP-32, Dopamine-and cAMP-regulated Neuronal Phosphoprotein, FLJ20940, Neuronal Phosphoprotein DARPP-32, Protein Phosphatase 1 Regulatory (Inhibitor) Subunit 1B, PPP1R1B) (FITC)
WB: 1ug/ml detects DARPP-32 on 10ug of rat brain tissue lysate.
Applications are based on unconjugated antibody.
Immunoprecipitation (IP)
(Immunoprecipitation Analysis: Representative lot data. 10 ug of MBS630808 immunoprecipitated DARPP-32 from 500 ug of rat brain RIPA lysate. Arrow indicates DARPP-32 (~32kDa).)
Immunoprecipitation (IP)
(Immunoprecipitation Analysis: Representative lot data. 10 ug of MBS630808 immunoprecipitated DARPP-32 from 500 ug of rat brain RIPA lysate. Arrow indicates DARPP-32 (~32kDa).)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. Rat brain tissue lysate was probed with D1075-01S(0.1 ug/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates DARPP-32 (~ 32 kDa).)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. Rat brain tissue lysate was probed with D1075-01S(0.1 ug/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates DARPP-32 (~ 32 kDa).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders in humans. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Uniprot Description
DARPP-32: a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32. Two alternatively-spliced isoforms have been described.
Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis
Cellular Component: cell soma; cytoplasm; intracellular; nucleus
Molecular Function: D4 dopamine receptor binding; protein phosphatase inhibitor activity; type 1 serine/threonine specific protein phosphatase inhibitor activity; D1 dopamine receptor binding; D3 dopamine receptor binding; D5 dopamine receptor binding; D2 dopamine receptor binding
Biological Process: negative regulation of female receptivity; transcription, DNA-dependent; response to amphetamine; negative regulation of catalytic activity; visual learning; negative regulation of protein kinase activity; signal transduction
Research Articles on DARPP32
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Product Notes
The DARPP32 ppp1r1b (Catalog #AAA6471913) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DARPP32 (Dopamine and cAMP-regulated Phosphoprotein DARPP32, DARPP 32, DARPP-32, Dopamine-and cAMP-regulated Neuronal Phosphoprotein, FLJ20940, Neuronal Phosphoprotein DARPP-32, Protein Phosphatase 1 Regulatory (Inhibitor) Subunit 1B, PPP1R1B) (FITC) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DARPP32 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). IP: 10ug immunoprecipitates DARPP-32 from rat brain RIPA lysate. WB: 1ug/ml detects DARPP-32 on 10ug of rat brain tissue lysate. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DARPP32 ppp1r1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DARPP32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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