Rabbit anti-Human, Mouse CCBP2 Polyclonal Antibody | anti-ACKR4 antibody

CCBP2, NT (Chemokine-binding Protein 2, Chemokine-binding Protein D6, C-C Chemokine Receptor D6, Chemokine Receptor CCR-9, CCR9, CC-Chemokine Receptor CCR-10, CCR10, CCBP2, CMKBR9)

Cat. Num. AAA647100

• Gene Names: ACKR4; PPR1; CCBP2; CCR10; CCR11; CCRL1; VSHK1; CCR-11; CKR-11; CCX CKR; CCX-CKR; CC-CKR-11
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: CCBP2; Polyclonal Antibody; NT (Chemokine-binding Protein 2; Chemokine-binding Protein D6; C-C Chemokine Receptor D6; Chemokine Receptor CCR-9; CCR9; CC-Chemokine Receptor CCR-10; CCR10; CMKBR9); Anti -CCBP2; anti-ACKR4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CCBP2. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-ACKR4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human CCBP2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CCBP2 Pab in mouse small intestine tissue lysate. CCBP2 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(CCBP2 Antibody (A313) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CCBP2 antibody detected the CCBP2 protein (arrow).)

Related Product Information for anti-ACKR4 antibody

CCBP2 is a beta chemokine receptor, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptor-mediated signal transduction are critical for the recruitment of effector immune cells to the inflammation site. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes.

Product Categories/Family for anti-ACKR4 antibody

Antibodies; Abs to G Protein Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 30795217
• NCBI GeneID: 51554
• NCBI Accession #: NP_848540.1
• NCBI GenBank Nucleotide #: NM_178445.2
• UniProt Accession #: Q9NPB9; B2R9U7
• Molecular Weight: 39,914 Da
• NCBI Official Full Name: C-C chemokine receptor type 11
• NCBI Official Synonym Full Names: atypical chemokine receptor 4
• NCBI Official Symbol: ACKR4
• NCBI Official Synonym Symbols: PPR1; CCBP2; CCR10; CCR11; CCRL1; VSHK1; CCR-11; CKR-11; CCX CKR; CCX-CKR; CC-CKR-11
• NCBI Protein Information: C-C chemokine receptor type 11; C-C CKR-11; CC chemokine receptor-like 1; chemocentryx chemokine receptor; chemokine (C-C motif) receptor-like 1; chemokine, cc motif, receptor-like protein 1; orphan seven-transmembrane receptor, chemokine related
• UniProt Protein Name: C-C chemokine receptor type 11
• Protein Family: Atypical chemokine receptor
• UniProt Gene Name: CCRL1
• UniProt Synonym Gene Names: CCBP2; CCR11; VSHK1; C-C CKR-11; CC-CKR-11; CCR-11; CCRL1
• UniProt Entry Name: CCRL1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the G protein-coupled receptor family, and is a receptor for C-C type chemokines. This receptor has been shown to bind dendritic cell- and T cell-activated chemokines including CCL19/ELC, CCL21/SLC, and CCL25/TECK. A pseudogene of this gene is found on chromosome 6. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2013]

Uniprot Description

Function: Receptor for CCL2, CCL8, CCL13, CCL19, CCL21 and CCL25. Ref.3

Subcellular location: Cell membrane; Multi-pass membrane protein.

Tissue specificity: Predominantly expressed in heart. Lower expression in lung, pancreas, spleen, colon, skeletal muscle and small intestine. Ref.1 Ref.2 Ref.7

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.