Rabbit DBC1 Polyclonal Antibody | anti-CCAR2 antibody

DBC1 (Protein KIAA1967, Deleted in Breast Cancer Gene 1 Protein, DBC-1, DBC.1, p30 DBC, KIAA1967)

Cat. Num. AAA647081

• Gene Names: CCAR2; DBC1; DBC-1; NET35; p30DBC; p30 DBC; KIAA1967
• Reactivity: Equine, Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: DBC1; Polyclonal Antibody; DBC1 (Protein KIAA1967; Deleted in Breast Cancer Gene 1 Protein; DBC-1; DBC.1; p30 DBC; KIAA1967); Anti -DBC1 (Protein KIAA1967; anti-CCAR2 antibody

• Host: Rabbit
• Reactivity: Equine, Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of total human DBC1. Species Crossreactivity: Equine, monkey, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-CCAR2 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50; Immunofluorescence: 1:100
• Immunogen: Synthetic peptide corresponding to residues near the amino terminus of human DBC1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from various cell lines using MBS647081.)

Related Product Information for anti-CCAR2 antibody

Deleted in breast cancer gene 1 protein (DBC1) was originally identified by its localization to a region of chromosome 8p21 that is homozygously deleted in breast cancer (1). DBC1 is a large, nuclear protein with multiple functions in cell survival. It binds directly to the estrogen receptor a (ERa) hormone-binding domain in a ligand-independent manner and may be a key determinant of ligand-independent ERa expression and survival in human breast cancer cells (2). DBC1 can promote p53-mediated apoptosis by binding to and inhibiting the deacetylase activity of SirT1, resulting in increased p53 acetylation levels and activity (3). DBC1 may be an important regulator of heterochromatin formation as it binds SUV39H1 and inhibits its histone methyltransferase activity (4). Caspase-dependent processing activates the pro-apoptotic activity of DBC1 during Tumor Necrosis Factor-a (TNF-a)-mediated cell death signaling (5). This processing of DBC1 in response to TNF-a is an early event in the onset of apoptosis and results in relocalization of DBC1 to the cytoplasm. Over-expression of the processed, cytoplasmic form of DBC1 results in mitochondrial clustering and matrix condensation and sensitizes cells to TNF-a-mediated apoptosis.

Product Categories/Family for anti-CCAR2 antibody

Antibodies; Abs to Oncoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 24432106
• NCBI GeneID: 57805
• NCBI Accession #: NP_066997.3
• NCBI GenBank Nucleotide #: NM_021174.5
• UniProt Accession #: Q8N163; Q6P0Q9; Q8N3G7; Q8N8M1; Q8TF34; Q9H9Q9; Q9HD12; Q9NT55; A6NL03; B2RB79; D3DSR6
• Molecular Weight: 102,902 Da
• NCBI Official Full Name: DBIRD complex subunit KIAA1967
• NCBI Official Synonym Full Names: cell cycle and apoptosis regulator 2
• NCBI Official Symbol: CCAR2
• NCBI Official Synonym Symbols: DBC1; DBC-1; NET35; p30DBC; p30 DBC; KIAA1967
• NCBI Protein Information: DBIRD complex subunit KIAA1967; p30 DBC protein; deleted in breast cancer 1
• UniProt Protein Name: DBIRD complex subunit KIAA1967
• Protein Family: Cell cycle and apoptosis regulator protein
• UniProt Gene Name: KIAA1967
• UniProt Synonym Gene Names: DBC1; DBC-1; DBC.1
• UniProt Entry Name: K1967_HUMAN

Uniprot Description

Function: Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. As part of a histone H3-specific methyltransferase complex may mediate ligand-dependent transcriptional activation by nuclear hormone receptors. Ref.9 Ref.10 Ref.13 Ref.14 Ref.20

Subunit structure: Component of the DBIRD complex. Interacts with ZNF326/ZIRD; the interaction is direct. Interacts (via N-terminus) with SIRT1. Interacts (via N-terminus) with SUV39H1; this interaction abolishes the interaction with SIRT1. Part of a complex composed at least of ASCL2, C11orf30/EMSY, HCFC1, HSPA8, KIAA1967, MATR3, MKI67,RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity. Ref.9 Ref.10 Ref.13 Ref.14 Ref.20

Subcellular location: Nucleus

Probable.

Tissue specificity: Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues. Ref.7

Sequence caution: The sequence AAG02472.1 differs from that shown. Reason: Frameshift at positions 610, 622, 794 and 810. The sequence BAB85553.1 differs from that shown. Reason: Erroneous initiation.