Rabbit anti-Human, Mouse Connexin 50 Polyclonal Antibody | anti-Cx50 antibody

Connexin 50, CT (Gap Junction alpha-8 Protein, GJA8, Connexin-50, Cx50, Lens Fiber Protein MP70) (MaxLight 490)

Cat. Num. AAA6470228

• Gene Names: GJA8; CAE; CAE1; CX50; CZP1; MP70; CTRCT1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Connexin 50; Polyclonal Antibody; CT (Gap Junction alpha-8 Protein; GJA8; Connexin-50; Cx50; Lens Fiber Protein MP70) (MaxLight 490); anti-Cx50 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Connexin 50. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-Cx50 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 402-433 in the C-terminal region of human Connexin 50, UniProt Accession #: P48165. Species sequence homology: chicken, sheep
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of mouse kidney tissue lysates (35ug/lane) usingMBS644692. GJA8 (arrow) was detected usingMBS644692.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue usingMBS644692, followed by secondary antibody DAB staining. This data demonstrates the use of MBS644692 for immunohistochemistry.)

Product Categories/Family for anti-Cx50 antibody

Antibodies; Connexin; Junction Proteins

References

1. Shiels, A., et al., Am. J. Hum. Genet. 62(3):526-532 (1998). 2. Church, R.L., et al., Curr. Eye Res. 14(10):979-981 (1995). 3. Church, R.L., et al., Curr. Eye Res. 14(3):215-221 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 55953076
• NCBI GeneID: 2703
• NCBI Accession #: NP_005258.2
• NCBI GenBank Nucleotide #: NM_005267.4
• UniProt Accession #: P48165; Q5VVN9; Q9NP25; A7L5M5
• Molecular Weight: 48,229 Da
• NCBI Official Full Name: gap junction alpha-8 protein
• NCBI Official Synonym Full Names: gap junction protein alpha 8
• NCBI Official Symbol: GJA8
• NCBI Official Synonym Symbols: CAE; CAE1; CX50; CZP1; MP70; CTRCT1
• NCBI Protein Information: gap junction alpha-8 protein
• UniProt Protein Name: Gap junction alpha-8 protein
• UniProt Gene Name: GJA8
• UniProt Synonym Gene Names: Cx50
• UniProt Entry Name: CXA8_HUMAN

NCBI Description

This gene encodes a transmembrane connexin protein that is necessary for lens growth and maturation of lens fiber cells. The encoded protein is a component of gap junction channels and functions in a calcium and pH-dependent manner. Mutations in this gene have been associated with zonular pulverulent cataracts, nuclear progressive cataracts, and cataract-microcornea syndrome. [provided by RefSeq, Dec 2009]