Western Blot (WB)
(Western blot analysis of Syntaphilin in human brain tissue lysate with Syntaphilin antibody at (A) 2 and (B) 4 μg/mL.)
Rabbit Syntaphilin Polyclonal Antibody | anti-SNPH antibody
Syntaphilin Antibody
Gene Names
SNPH; bA314N13.5
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Syntaphilin Antibody is affinity chromatography purified via peptide column.
Synonyms
Syntaphilin; Polyclonal Antibody; Syntaphilin Antibody; bA314N13.5; KIAA0374; syntaphilin; anti-SNPH antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Syntaphilin Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
538
Applicable Applications for anti-SNPH antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Syntaphilin antibody can be used for detection of Syntaphilin by Western blot at 2 - 4 mug/mL. Despite its predicted molecular weight, Syntaphilin usually migrates at higher molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
Syntaphilin antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human Syntaphilin.
Buffer
Syntaphilin Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
Syntaphilin antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of Syntaphilin in human brain tissue lysate with Syntaphilin antibody at (A) 2 and (B) 4 μg/mL.)
Western Blot (WB)
(Western blot analysis of Syntaphilin in human brain tissue lysate with Syntaphilin antibody at (A) 2 and (B) 4 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of Syntaphilin in human brain with Syntaphilin antibody at 5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of Syntaphilin in human brain with Syntaphilin antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of Syntaphilin in Human Brain cells with Syntaphilin antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of Syntaphilin in Human Brain cells with Syntaphilin antibody at 20 μg/mL.)
Related Product Information for anti-SNPH antibody
Syntaphilin Antibody: Syntaphilin was initially identified in a yeast two-hybrid screen with the carboxy terminal region of Syntaxin-1 as bait. Syntaxin-1 is a key component of the synaptic vesicle docking machinery that forms the SNARE complex with synaptobrevin and SNAP-25. Syntaphilin competes with SNAP-25 for binding to syntaxin-1 and inhibits the formation of the SNARE complex, thereby potentially regulating synaptic vesicle exocytosis. Syntaphilin also binds dynamin-1 and inhibits dynamin-dependent endocytosis. Mice lacking syntaphilin show an increased level of mitochondrial motility and a reduced density of axonal mitochondria. This correlates with an enhanced short-term facilitation and significant impairments in motor ability, suggesting syntaphilin plays a major role in presynaptic function. Multiple isoforms are known to exist.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,990 Da
NCBI Official Full Name
Syntaphilin
NCBI Official Synonym Full Names
syntaphilin
NCBI Official Symbol
SNPH
NCBI Official Synonym Symbols
bA314N13.5
NCBI Protein Information
syntaphilin
UniProt Protein Name
Syntaphilin
Protein Family
UniProt Gene Name
SNPH
UniProt Synonym Gene Names
KIAA0374
UniProt Entry Name
SNPH_HUMAN
NCBI Description
Syntaxin-1, synaptobrevin/VAMP, and SNAP25 interact to form the SNARE complex, which is required for synaptic vesicle docking and fusion. The protein encoded by this gene is membrane-associated and inhibits SNARE complex formation by binding free syntaxin-1. Expression of this gene appears to be brain-specific. [provided by RefSeq, Jul 2008]
Uniprot Description
syntaphilin: Inhibits SNARE complex formation by absorbing free syntaxin-1. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle; Membrane protein, integral
Chromosomal Location of Human Ortholog: 20p13
Cellular Component: presynaptic membrane; neuron projection; cell soma; cytoplasmic microtubule; cytoplasm; mitochondrial membrane; integral to membrane; cell junction
Molecular Function: syntaxin-1 binding
Biological Process: neuron differentiation; neurotransmitter secretion; brain development; synaptic vesicle docking during exocytosis
Research Articles on SNPH
Similar Products
Product Notes
The SNPH snph (Catalog #AAA150160) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Syntaphilin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Syntaphilin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Syntaphilin antibody can be used for detection of Syntaphilin by Western blot at 2 - 4 mug/mL. Despite its predicted molecular weight, Syntaphilin usually migrates at higher molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the SNPH snph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Syntaphilin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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