Rabbit anti-Human IGF2BP2 Polyclonal Antibody | anti-IGF2BP2 antibody

IGF2BP2, CT (IGF2BP2, IMP2, VICKZ2, Insulin-like growth factor 2 mRNA-binding protein 2, Hepatocellular carcinoma autoantigen p62, IGF-II mRNA-binding protein 2, VICKZ family member 2)

Cat. Num. AAA646977

• Gene Names: IGF2BP2; p62; IMP2; IMP-2; VICKZ2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: IGF2BP2; Polyclonal Antibody; CT (IGF2BP2; IMP2; VICKZ2; Insulin-like growth factor 2 mRNA-binding protein 2; Hepatocellular carcinoma autoantigen p62; IGF-II mRNA-binding protein 2; VICKZ family member 2); Anti -IGF2BP2; anti-IGF2BP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-IGF2BP2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: IGF2BP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 537-564 amino acids from the C-terminal region of human IGF2BP2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane) using MBS646977. This demonstrates that MBS646977 detected the IGF2BP2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue using MBS646977 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646977 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HepG2 cells using MBS646977 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-IGF2BP2 antibody

This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Categories/Family for anti-IGF2BP2 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 64085377
• NCBI GeneID: 10644
• NCBI Accession #: NP_006539.3
• NCBI GenBank Nucleotide #: NM_006548.4
• UniProt Accession #: Q9Y6M1; A0A4Z0; B3FTN2; B3FTN3; B3FTN4
• Molecular Weight: 66,121 Da
• NCBI Official Full Name: insulin-like growth factor 2 mRNA-binding protein 2 isoform a
• NCBI Official Synonym Full Names: insulin-like growth factor 2 mRNA binding protein 2
• NCBI Official Symbol: IGF2BP2
• NCBI Official Synonym Symbols: p62; IMP2; IMP-2; VICKZ2
• NCBI Protein Information: insulin-like growth factor 2 mRNA-binding protein 2; VICKZ family member 2; IGF2 mRNA-binding protein 2; IGF-II mRNA-binding protein 2; hepatocellular carcinoma autoantigen p62
• UniProt Protein Name: Insulin-like growth factor 2 mRNA-binding protein 2
• Protein Family: Insulin-like growth factor 2 mRNA-binding protein
• UniProt Gene Name: IGF2BP2
• UniProt Synonym Gene Names: IMP2; VICKZ2; IGF2 mRNA-binding protein 2; IMP-2
• UniProt Entry Name: IF2B2_HUMAN

NCBI Description

This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation

By similarity. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts. Ref.5 Ref.17

Subunit structure: Can form homooligomers and heterooligomers with IGF2BP1 and IGF2BP3 in an RNA-dependent manner. Interacts with HNRPD and IGF2BP1. Interacts with ELAVL1, DHX9 and HNRNPU. Ref.6 Ref.9 Ref.17

Subcellular location: Nucleus. Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. In response to cellular stress, such as oxidative stress, recruited to stress granules. Ref.1 Ref.5 Ref.8 Ref.9 Ref.17

Tissue specificity: Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis. Ref.1 Ref.8

Domain: Domain KH3 and KH4 are the major RNA-binding modules, although KH1 and KH2 may also contribute. The contribution to RNA-binding of individual KH domains may be target-specific. KH1 and KH2, and possibly KH3 and KH4, promote the formation of higher ordered protein-RNA complexes, which may be essential for IGF2BP1 cytoplasmic retention. KH domains are required for RNA-dependent homo- and heterooligomerization and for localization to stress granules. Ref.13 Ref.17

Miscellaneous: Autoantibodies against IGF2BP2 are detected in sera from some patients with hepatocellular carcinoma.

Sequence similarities: Belongs to the RRM IMP/VICKZ family.Contains 4 KH domains.Contains 2 RRM (RNA recognition motif) domains.

Sequence caution: The sequence AAH21290.1 differs from that shown. Reason: Erroneous initiation.