Rabbit anti-Human CD117 Polyclonal Antibody | anti-CD117 antibody

CD117 (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF) (BSA & Azide Free) (AP)

Cat. Num. AAA6468214

• Gene Names: KIT; PBT; SCFR; C-Kit; CD117
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD117; Polyclonal Antibody; CD117 (c-kit; Stem Cell Factor; SCF Receptor; Steel Factor Receptor; Mast Cell Growth Factor; MGF) (BSA & Azide Free) (AP); anti-CD117 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CD117 at 145kD.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CD117 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: IHC-P: 1:400-1:800 for 30 min at RT. Excellent for staining of formalin-fixed, paraffin-embedded tissues.; Applications are based on unconjugated antibody.
• Immunogen: A synthetic peptide from C-terminus of human CD117/c-kit protein
• Conjugate: AP
• Positive Control: Tonsil, GI Stromal Tumor (GIST).
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Categories/Family for anti-CD117 antibody

Antibodies; CD Markers

References

1. Arber D A, et al. Hum. Pathol. 29(5): 498-504 (1998). 2. Rajpert-De Meyts E, et al. Journal of Pathology. 178: 166-169(1996). Additional: 1. Besmer P, Murphy JE, George PC, Qiu F, Bergold PJ, Lederman L, Snyder HW Jr, Brodeur D, Zuckerman EE and Hardy WD (1986) A new acute transforming feline retrovirus and relationship of its oncogene v-kit with the protein kinase family. Nature 320, 415-421. 2. Yarden Y, Kuang W-J, Yang-Feng T, Coussens L, Munemitsu S, Dull TJ, Chen E, Schlessinger J, Francke U and Ullrich A (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J 6, 3341-3351. 3. Chabot B, Stephenson DA, Chapman VM, Besmer P and Bernstein A (1988) The proto-oncogene c-kit encoding a transmembrane tyrosine kinase receptor maps to the mouse W locus. Nature 335, 88-89. 4. Geissler EN, Ryan MA and Housman DE (1988) The dominant white spotting (W) locus of the mouse encodes the c-kit proto-oncogene. Cell 55, 185-192. 5. Hoo JJ, Haslam RHA and van Orman (1986) Tentative assignment of piebald trait gene to chromosome band 4q12. Hum Genet 73, 230-231. 6. Qiu F, Ray P, Brown K, Barker PE, Jhanwar S, Ruddle FH and Besmer P (1988) Primary structure of c-kit: relationship with the CSF-1/PDGF receptor kinase family-oncogenic activation of v-kit involves deletion of extracellular domain and C terminus. EMBO J 7, 1003-1011. 7. Russell ES (1949) Analysis of pleiotropism at the W locus in the mouse: Relationship between the effects of W and WV substitution on hair pigmentation and on erythrocytes. Genetics 34, 398-402. 8. Sarvella PA and Russell ES (1956) Steel, a new dominant gene in the house mouse. J Hered 47, 123-128. 9. Ebbe S, Phalen E and Stolman FJ (1973) Abnormal megakaryocytopoiesis in W/WV mice. and: Abnormal megakaryocytopoiesis in Sl/Sld mice. Blood 42, 857-864 and 865-871. 10. Nishimune Y, Haneji T and Kitamura Y (1980) The effects of Steel mutation on testicular germ cell differentiation. J Cell Physiol 105, 137-141. 11. Morrison-Graham K and Weston JA (1989) Mouse mutants provide new insights into the role of extracellular matrix in cell migration and differentiation. Trends Genet 5, 116-121. 12. Harrison DE and Russell ES (1972) The response of W/WV and Sl/Sld anemic mice to haematopoietic stimuli. Br J Haematol 22, 155-168. 13. Kitamura Y, Go S and Hatanaka K (1978) Decrease of mast cells in W/WV mice and their increase by bone marrow transplantation. Blood 52, 447-452. 14. Jarboe DJ and Huff TF (1989) W/WV mice do not make mast cell-committed progenitors and Sl/Sld fibroblasts do not support development of normal mast cell committed progenitors. J Immunol 7, 2418-2423. 15. Williams DE, Eisenman J, Baird A, Rauch C, van Ness K, March CJ, Park LS, Martin U, Mochizuki DY, Boswell HS, Burgess GS, Cosman D and Lyman SD (1990) Identification of a ligand for the c-kit proto-oncogene. Cell 63, 167-174. 16. Zsebo KM, Wypych J, McNiece IK, Lu HS, Smith KA, Karkare S, Sachdev RK; Yuschenkoff VN, Birkett NC, Williams LR, Satyagal VN, Tung W, Bosselman RA, Mendiaz EA and Langley KE (1990) Identification, purification and biological characterization of stem cell factor from Buffalo rat liver conditioned medium. Cell 63, 195-201. 17. Martin FH, Suggs SV, Langley KE, Lu HS, Ting J, Okino KH, Morris FH, McNiece IK, Jacobsen RW, Mendiaz EA, Birkett NC, Smith KA, Johnson MJ, Parker VP, Flores JC; Patel AC, Fisher EF, Erjavec HO, Wypych J, Sachdev RK, Pope JA, Leslie I, Wen D, Lin CH, Cupples RL and Zsebo KM (1990) Primary structure and functional expression of rat and human stem cell factor. Cell 63, 203-211. 18. Zsebo KM, Williams DA, Geissler EN, Broudy VC, Martin FH, Atkins HL, Hsu R-Y, Birkett NC, Okino KH, Murdock DC, Jacobsen RW, Langley KE, Smith KA, Takeishi T, Cattanach BM, Galli SJ and Suggs SV (1990) Stem cell factor is encoded at the Sl locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor. Cell 63, 213-224. 19. Huang E, Nocka K, Beier D, Chu T-Y, Buck J, Lahm H-W, Wellner D, Leder P and Besmer P (1990) The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor, the gene product of the W locus. Cell 63, 225-233. 20. Anderson DM, Lyman SD, Baird A, Wignall JM, Eisenman J, Rauch C, March CJ, Boswell HS, Gimpel SD, Cosman D and Williams DE (1990) Molecular cloning of mast cell growth factor, a hematopoietin that is active in both membrane bound and soluble forms. Cell 63, 235-243. 21. Copeland NG, Gilbert DJ, Cho BC, Donovan PJ, Jenkins NA, Cosman D, Anderson DM, Lyman SD and Williams DE (1990) Mast cell growth factor maps near the Sl locus on mouse chromosome 10 and is deleted in a number of Steel alleles. Cell 63, 175-183. 22. Flanagan JG, Chan DC and Leder P (1991) Transmembrane form of the kit ligand growth factor is determined by alternative splicing and is missing in the Sld mutant. Cell 64, 1025-1035. 23. Toksoz D, Zsebo KM, Smith KA, Hu S, Brankow D, Suggs SV, Martin FH and Wiliams DA (1992) Support of human hematopoiesis in long-term bone marrow culture by murine stromal cells selectively expressing the membrane-bound and secreted forms of the human homolog of the steel gene product, stem cell factor. Proc Natl Acad Sci USA 89, 7350-7354. 24. Nocka K, Majumder S, Chabot B, Ray P, Cervon M, Bernstein A and Besmer P (1989) Expression of c-kit gene products in known cellular targets of W mutations in normal and W mutant mice, evidence for an impaired c-kit kinase in mutant mice. Genes Dev 3, 816-826.

NCBI and Uniprot Product Information

• NCBI GI #: 4557695
• NCBI GeneID: 3815
• NCBI Accession #: NP_000213.1; BC071593
• NCBI GenBank Nucleotide #: NM_000222.2
• UniProt Accession #: P10721; Q6IQ28; Q99662; Q9UM99; B5A956; D5LXN2; D5M931; F5H8F8
• Molecular Weight: 109,865 Da
• NCBI Official Full Name: mast/stem cell growth factor receptor Kit isoform 1
• NCBI Official Synonym Full Names: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• NCBI Official Symbol: KIT
• NCBI Official Synonym Symbols: PBT; SCFR; C-Kit; CD117
• NCBI Protein Information: mast/stem cell growth factor receptor Kit; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like
• UniProt Protein Name: Mast/stem cell growth factor receptor Kit
• UniProt Gene Name: KIT
• UniProt Synonym Gene Names: SCFR; SCFR; PBT
• UniProt Entry Name: KIT_HUMAN

NCBI Description

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Kit: a receptor tyrosine kinase and a member of the subfamily that includes PDGF, CSF-1 and FLT-3/flk-2 receptors. Receptor for stem cell factor. Plays a critical role in hematopoietic stem cell, mast cell, melanocyte and germ cell development. Ligand binding induces autophosphorylation, dimerization and activation, leading to the recruitment and phosphorylation of downstream SH2-containing signaling components including PLC-gamma, PI3 kinase p85, SHP2 and CrkL, linking c-Kit to various cell signaling pathways. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders, while mutations that constitutively activate c-Kit can lead to hyperplasia and tumorigenesis. Activating mutations cause >90% of gastrointestinal stromal tumors (GIST); successfully treated with inhibitors Gleevec (imatinib, Glivec) and Sutent (Sutinib, SU11248). Activating mutations also induce mastocytosis. Autocrine/paracrine stimulation may drive some lung and other tumors. Loss of expression associated with melanoma progression. Familial loss of function mutations cause piebaldism, with defects in hair and skin pigmentation due to lack of melanocytes.

Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, TK; Oncoprotein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; PDGFR family

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: extracellular space; internal side of plasma membrane; mast cell granule; acrosome; plasma membrane; integral to membrane; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; protease binding; metal ion binding; protein-tyrosine kinase activity; cytokine binding; stem cell factor receptor activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; somatic stem cell maintenance; germ cell programmed cell death; positive regulation of JAK-STAT cascade; lymphoid progenitor cell differentiation; positive regulation of long-term neuronal synaptic plasticity; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of cell shape; epithelial cell proliferation; germ cell migration; erythrocyte differentiation; somatic stem cell division; T cell differentiation; fibroblast growth factor receptor signaling pathway; embryonic hemopoiesis; mast cell chemotaxis; stem cell differentiation; detection of mechanical stimulus involved in sensory perception of sound; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of tyrosine phosphorylation of Stat1 protein; immature B cell differentiation; positive regulation of transcription factor activity; regulation of pigmentation during development; glycosphingolipid metabolic process; mast cell cytokine production; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; signal transduction; myeloid progenitor cell differentiation; ovarian follicle development; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; melanocyte differentiation; negative regulation of programmed cell death; visual learning; hemopoiesis; inflammatory response; positive regulation of Notch signaling pathway; epidermal growth factor receptor signaling pathway; lamellipodium biogenesis; phosphoinositide-mediated signaling; dendritic cell cytokine production; cytokine and chemokine mediated signaling pathway; male gonad development; stem cell maintenance; positive regulation of phosphoinositide 3-kinase activity; mast cell degranulation; regulation of cell proliferation; positive regulation of pseudopodium formation; gut development; positive regulation of tyrosine phosphorylation of Stat5 protein; pigmentation; actin cytoskeleton reorganization; innate immune response; spermatogenesis; spermatid development; positive regulation of cell migration

Disease: Gastrointestinal Stromal Tumor; Mast Cell Disease; Piebald Trait; Testicular Germ Cell Tumor