Rabbit anti-Human, Mouse CPE Polyclonal Antibody | anti-CPE antibody

CPE, CT (Carboxypeptidase E, CPE, Carboxypeptidase H, CPH, Enkephalin Convertase, Prohormone-processing Carboxypeptidase) (PE)

Cat. Num. AAA6467107

• Gene Names: CPE; CPH
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CPE; Polyclonal Antibody; CT (Carboxypeptidase E; Carboxypeptidase H; CPH; Enkephalin Convertase; Prohormone-processing Carboxypeptidase) (PE); anti-CPE antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CPE. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CPE antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CPE (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CPE antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane). CPE (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of CPE antibody (C-term) in Hela cell line lysates (35ug/lane). CPE (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-CPE antibody

Antibodies; Enzymes; Carboxypeptidase

References

1. Oiso, S., J. Neurochem. 109(1), 158-167 (2009). 2. Yang, L., Ann. N. Y. Acad. Sci. 1150, 263-266 (2008). 3. Jeffrey, K.D., Proc. Natl. Acad. Sci. USA 105(24), 8452-8457 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4503009
• NCBI GeneID: 1363
• NCBI Accession #: NP_001864
• NCBI GenBank Nucleotide #: NM_001873.3
• UniProt Accession #: P16870; Q9UIU9; A8K4N1; B3KR42; B4DFN4; D3DP33
• Molecular Weight: 51.4 kDa (457aa)
• NCBI Official Full Name: carboxypeptidase E preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase E
• NCBI Official Symbol: CPE
• NCBI Official Synonym Symbols: CPH
• NCBI Protein Information: carboxypeptidase E
• UniProt Protein Name: Carboxypeptidase E
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPE
• UniProt Synonym Gene Names: CPE; CPH
• UniProt Entry Name: CBPE_HUMAN

NCBI Description

This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes. [provided by RefSeq, Nov 2015]

Uniprot Description

CPE: Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Protease; EC 3.4.17.10; Secreted

Chromosomal Location of Human Ortholog: 4q32.3

Cellular Component: Golgi apparatus; secretory granule membrane; plasma membrane; nucleus

Molecular Function: carboxypeptidase activity; zinc ion binding; neurexin binding; cell adhesion molecule binding; metallocarboxypeptidase activity

Biological Process: insulin processing; cellular protein metabolic process; metabolic process; neuropeptide signaling pathway; protein modification process; proteolysis