Rabbit anti-Human CTSK Polyclonal Antibody | anti-CTSK antibody

CTSK, ID (E112) (CTSK, CTSO, CTSO2, Cathepsin K, Cathepsin O, Cathepsin O2, Cathepsin X)

Cat. Num. AAA642024

• Gene Names: CTSK; CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CTSK; Polyclonal Antibody; ID (E112) (CTSK; CTSO; CTSO2; Cathepsin K; Cathepsin O; Cathepsin O2; Cathepsin X); Anti -CTSK; anti-CTSK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-CTSK antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:50-100; Immunohistochemistry: 1:10-50; Flow Cytometry: 1:10-50
• Immunogen: CTSK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-126 amino acids from the Central region of human CTSK.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CTSK (arrow) using rabbit polyclonal CTSK Antibody (Center E112) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTSK gene.)

Immunohistochemistry (IHC)

(CTSK Antibody (Center E112) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CTSK Antibody (Center E112) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CTSK Antibody (Center E112) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CTSK antibody

The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing. [provided by RefSeq].

Product Categories/Family for anti-CTSK antibody

Antibodies; Abs to Cathepsin

NCBI and Uniprot Product Information

• NCBI GI #: 4503151
• NCBI GeneID: 1513
• NCBI Accession #: NP_000387.1
• NCBI GenBank Nucleotide #: NM_000396.3
• UniProt Accession #: P43235; Q6FHS6
• Molecular Weight: 36,966 Da
• NCBI Official Full Name: cathepsin K preproprotein
• NCBI Official Synonym Full Names: cathepsin K
• NCBI Official Symbol: CTSK
• NCBI Official Synonym Symbols: CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2
• NCBI Protein Information: cathepsin K; cathepsin O; cathepsin X; cathepsin O1; cathepsin O2
• UniProt Protein Name: Cathepsin K
• Protein Family: Cathepsin
• UniProt Gene Name: CTSK
• UniProt Synonym Gene Names: CTSO; CTSO2
• UniProt Entry Name: CATK_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. [provided by RefSeq, Apr 2013]

Uniprot Description

CTSK: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Defects in CTSK are the cause of pycnodysostosis (PKND). PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.38; Protease

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; extracellular region

Molecular Function: collagen binding; protein binding; proteoglycan binding; fibronectin binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: collagen catabolic process; extracellular matrix disassembly; intramembranous ossification; extracellular matrix organization and biogenesis; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; proteolysis; bone resorption

Disease: Pycnodysostosis