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Western Blot (WB) (Western blot analysis of anti-CAMK 1G Pab in NCI-H460 cell lysate. CAMK 1G (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Rabbit anti-Human CAMK1G Polyclonal Antibody | anti-CAMK1G antibody

CAMK1G, CT (Calcium/Calmodulin-dependent Protein Kinase IG, CaM Kinase I gamma, CaM Kinase IG, CaM-KI gamma, CaMKI gamma, CaMKIG, CaMK-like CREB Kinase III, CLICK III, VWS1) (Azide free) (HRP)

Gene Names
CAMK1G; VWS1; CLICK3; CLICKIII; dJ272L16.1
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
CAMK1G; Polyclonal Antibody; CT (Calcium/Calmodulin-dependent Protein Kinase IG; CaM Kinase I gamma; CaM Kinase IG; CaM-KI gamma; CaMKI gamma; CaMKIG; CaMK-like CREB Kinase III; CLICK III; VWS1) (Azide free) (HRP); anti-CAMK1G antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CaMKIG.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-CAMK1G antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human CaMKIG (KLH).
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CAMK 1G Pab in NCI-H460 cell lysate. CAMK 1G (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB) (Western blot analysis of anti-CAMK 1G Pab in NCI-H460 cell lysate. CAMK 1G (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB)

(Western blot analysis of CAMK1G (arrow) using rabbit polyclonal CAMK1G Antibody (C-term) (RB01249). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1G gene (Lane 2) (Origene Technologies).)

Western Blot (WB) (Western blot analysis of CAMK1G (arrow) using rabbit polyclonal CAMK1G Antibody (C-term) (RB01249). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1G gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Product Categories/Family for anti-CAMK1G antibody
References
1. Takemoto-Kimura, S., et al., J. Biol. Chem. 278(20):18597-18605 (2003). 2. Schutte, B.C., et al., Genome Res. 10(1):81-94 (2000).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,486 Da
NCBI Official Full Name
calcium/calmodulin-dependent protein kinase type 1G
NCBI Official Synonym Full Names
calcium/calmodulin-dependent protein kinase IG
NCBI Official Symbol
CAMK1G
NCBI Official Synonym Symbols
VWS1; CLICK3; CLICKIII; dJ272L16.1
NCBI Protein Information
calcium/calmodulin-dependent protein kinase type 1G; CLICK III; caM kinase I gamma; caM kinase IG; caM-KI gamma; caMK-like CREB kinase III; caMKI gamma; caMKIG
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase type 1G
UniProt Gene Name
CAMK1G
UniProt Synonym Gene Names
CLICK3; VWS1; CaM kinase IG; CaM-KI gamma; CaMKI gamma; CaMKIG; CLICK III
UniProt Entry Name
KCC1G_HUMAN

Uniprot Description

CAMK1G: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.17; CAMK group; CAMK1 family

Chromosomal Location of Human Ortholog: 1q32.2

Cellular Component: Golgi membrane; neuron projection; plasma membrane; calcium- and calmodulin-dependent protein kinase complex

Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: protein amino acid phosphorylation

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Product Notes

The CAMK1G camk1g (Catalog #AAA6466771) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CAMK1G, CT (Calcium/Calmodulin-dependent Protein Kinase IG, CaM Kinase I gamma, CaM Kinase IG, CaM-KI gamma, CaMKI gamma, CaMKIG, CaMK-like CREB Kinase III, CLICK III, VWS1) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CAMK1G can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CAMK1G camk1g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAMK1G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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