Rabbit anti-Human Bik Polyclonal Antibody | anti-Bik antibody

Bik, Phosphorylated (Thr33) (Bcl-2 Interacting Killer, Apoptosis Inducer NBK, BP4, BIP1) (PE)

Cat. Num. AAA6465342

• Gene Names: BIK; BP4; NBK; BIP1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Bik; Polyclonal Antibody; Phosphorylated (Thr33) (Bcl-2 Interacting Killer; Apoptosis Inducer NBK; BP4; BIP1) (PE); anti-Bik antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Bik when phosphorylated at Thr33.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Bik antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr33 of human Bik (KLH).
• Conjugate: PE
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-Bik Pab in mouse liver tissue lysate (35ug/lane). Bik(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-Bik antibody

Antibodies; Apoptosis

References

1. Nikrad, M., et al., Mol. Cancer Ther. 4(3):443-449 (2005). 2. Dong, F., et al., Infect. Immun. 73(3):1861-1864 (2005). 3. Hur, J., et al., Proc. Natl. Acad. Sci. USA 101(8):2351-2356 (2004). 4. Gillissen, B., et al., EMBO J. 22(14):3580-3590 (2003). 5. Arena, V., et al., Genes Chromosomes Cancer 38(1):91-96 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4502411
• NCBI GeneID: 638
• NCBI Accession #: NP_001188
• NCBI GenBank Nucleotide #: NM_001197.5
• UniProt Accession #: Q13323
• Molecular Weight: 17kDa
• NCBI Official Full Name: bcl-2-interacting killer
• NCBI Official Synonym Full Names: BCL2 interacting killer
• NCBI Official Symbol: BIK
• NCBI Official Synonym Symbols: BP4; NBK; BIP1
• NCBI Protein Information: bcl-2-interacting killer
• UniProt Protein Name: Bcl-2-interacting killer
• Protein Family: Bcl-2-interacting killer
• UniProt Gene Name: BIK
• UniProt Synonym Gene Names: NBK
• UniProt Entry Name: BIK_HUMAN

NCBI Description

The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins. [provided by RefSeq, Sep 2011]

Uniprot Description

BIK: accelerates programmed cell death. Binding to the apoptosis repressors Bcl-xL, Bcl-2, or BHRF1 (the Epstein-Barr virus homolog of Bcl-2) suppresses its death-promoting activity. Contains 1 Bcl-2 homology 3 (BH3) domain. Does not interact with Bax.

Protein type: Apoptosis; Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: mitochondrial membrane; integral to membrane; endomembrane system

Molecular Function: BH domain binding; protein binding; protein heterodimerization activity

Biological Process: apoptotic mitochondrial changes; positive regulation of protein homooligomerization; apoptosis; male gonad development; spermatogenesis