Rabbit anti-Rat Aquaporin 1 Polyclonal Antibody | anti-AQP1 antibody

Aquaporin 1 (AQP1, CHIP28) (APC)

Cat. Num. AAA6463877

• Gene Names: AQP1; CO; CHIP28; AQP-CHIP
• Reactivity: Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified by Immunoaffinity chromatography
• Synonyms: Aquaporin 1; Polyclonal Antibody; Aquaporin 1 (AQP1; CHIP28) (APC); anti-AQP1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Aquaporin 1 at ~28.8kD. Species Sequence Homology: human, mouse, bovine, sheep: 100%
• Purity/Purification: Purified by Immunoaffinity chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 2754

• Applicable Applications for anti-AQP1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to 19aa from the cytosolic carboxyl terminal domain of the AQP1, conjugated to KLH. This peptide contains most of the epitopes recognized by polyclonal antibodies against the whole protein (NP_036910.1).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot: A3000 (0.5ug/ml) in adult rat heart lysate.)

Related Product Information for anti-AQP1 antibody

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Aquaporin, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells. Over the last decade, genes for several members of the aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin 1 (APQ1) is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues.

Product Categories/Family for anti-AQP1 antibody

Antibodies; Aquaporin

References

1. Mobasheri, A., et al., Vet. J. 168: 143-150 (2004). 2. Mobasheri, A., et al., BJU International 92: 793-802 (2003). 3. Gallardo, P., et al., Am. J. Physiol. Regul. Integr. Comp. Physiol. 283: R779-R788 (2002). 4. Zhou, Q., et al., PNAS 98(24): 14, 132-14, 137 (2001). 5. Belyantseva, I.A., et al., J. Neurosci. 20(24): 8996-9003 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 1813789242
• NCBI GeneID: 358
• NCBI Accession #: NM_198098.4
• NCBI GenBank Nucleotide #: NM_198098
• UniProt Accession #: P29972
• NCBI Official Full Name: Homo sapiens aquaporin 1 (Colton blood group) (AQP1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: aquaporin 1 (Colton blood group)
• NCBI Official Symbol: AQP1
• NCBI Official Synonym Symbols: CO; CHIP28; AQP-CHIP
• NCBI Protein Information: aquaporin-1
• UniProt Protein Name: Aquaporin-1
• UniProt Gene Name: AQP1
• UniProt Synonym Gene Names: CHIP28; AQP-1
• UniProt Entry Name: AQP1_HUMAN

NCBI Description

This gene encodes a small integral membrane protein with six bilayer spanning domains that functions as a water channel protein. This protein permits passive transport of water along an osmotic gradient. This gene is a possible candidate for disorders involving imbalance in ocular fluid movement. [provided by RefSeq, Aug 2016]

Uniprot Description

AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter, aquaporin family; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: nuclear membrane; integral to plasma membrane; basolateral plasma membrane; brush border membrane; apical part of cell; apical plasma membrane; cytoplasm; plasma membrane; basal plasma membrane; nerve terminal; nucleus; sarcolemma; brush border

Molecular Function: protein binding; ephrin receptor binding; potassium channel activity; water transporter activity; intracellular cGMP activated cation channel activity; ammonium transmembrane transporter activity; glycerol transmembrane transporter activity; potassium ion transmembrane transporter activity; nitric oxide transporter activity; water channel activity; transmembrane transporter activity; glycerol channel activity

Biological Process: cGMP biosynthetic process; glycerol transport; cerebrospinal fluid secretion; wound healing; cellular response to stress; water transport; carbon dioxide transport; negative regulation of caspase activity; pancreatic juice secretion; sensory perception of pain; positive regulation of saliva secretion; odontogenesis; positive regulation of fibroblast proliferation; hyperosmotic salinity response; nitric oxide transport; bicarbonate transport; multicellular organismal water homeostasis; cellular homeostasis; transmembrane transport; potassium ion transport; lipid digestion; lateral ventricle development; response to drug; cellular water homeostasis; secretory granule organization and biogenesis; glomerular filtration; ammonium transport; renal water transport; positive regulation of angiogenesis; camera-type eye morphogenesis; adrenocorticotropin hormone secretion; response to estrogen stimulus; establishment and/or maintenance of actin cytoskeleton polarity; renal water homeostasis; cell volume homeostasis; negative regulation of apoptosis

Disease: Blood Group--colton