Rabbit anti-Human, Mouse AK3 Polyclonal Antibody | anti-AK3 antibody

AK3, CT (H38) (Adenylate Kinase Isoenzyme 4, Mitochondrial, Adenylate Kinase 3-like, ATP-AMP Transphosphorylase, AK3L1, AK3L2, AK4) (PE)

Cat. Num. AAA6461663

• Gene Names: AK4; AK3; AK 4; AK3L1; AK3L2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: AK3; Polyclonal Antibody; CT (H38) (Adenylate Kinase Isoenzyme 4; Mitochondrial; Adenylate Kinase 3-like; ATP-AMP Transphosphorylase; AK3L1; AK3L2; AK4) (PE); anti-AK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AK3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-AK3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human AK3 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(The anti-AK3 Pab is used in Western blot to detect AK3 in mouse kidney tissue lysate.)

Western Blot (WB)

(Western blot analysis of AK3 (arrow) using rabbit polyclonal AK3 Antibody (C-term H38). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AK3 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-AK3 antibody

Antibodies; Enzymes

References

1. Van Rompay, A.R., et al., Eur. J. Biochem. 261(2):509-517 (1999). 2. Yoneda, T., et al., Brain Res. Mol. Brain Res. 62(2):187-195 (1998). 3. Xu, G., et al., Genomics 13(3):537-542 (1992). 4. Robson, E.B., et al., Cytogenet. Cell Genet. 32(1-4), 144-152 (1982).

NCBI and Uniprot Product Information

• NCBI GI #: 8051579
• NCBI GeneID: 205
• NCBI Accession #: NP_037542; NP_001005353; NP_982289
• NCBI GenBank Nucleotide #: NM_013410.3
• UniProt Accession #: P27144; Q6IBH4; Q6NXQ5; Q8IUU9; B2R927; D3DQ62
• Molecular Weight: 25,268 Da
• NCBI Official Full Name: adenylate kinase 4, mitochondrial
• NCBI Official Synonym Full Names: adenylate kinase 4
• NCBI Official Symbol: AK4
• NCBI Official Synonym Symbols: AK3; AK 4; AK3L1; AK3L2
• NCBI Protein Information: adenylate kinase 4, mitochondrial
• UniProt Protein Name: Adenylate kinase 4, mitochondrial
• Protein Family: GTP:AMP phosphotransferase
• UniProt Gene Name: AK4
• UniProt Entry Name: KAD4_HUMAN

NCBI Description

This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq, Jul 2008]

Uniprot Description

AK3L1: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. May also be active with GTP. Belongs to the adenylate kinase family.

Protein type: Nucleotide Metabolism - purine; EC 2.7.4.10; EC 2.7.4.6; Mitochondrial; Kinase, other

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: GTP binding; nucleoside diphosphate kinase activity; adenylate kinase activity; nucleoside triphosphate adenylate kinase activity; ATP binding

Biological Process: response to drug; ATP metabolic process; nucleoside triphosphate biosynthetic process; nucleoside diphosphate phosphorylation; ADP biosynthetic process; brain development; liver development; AMP metabolic process; GTP metabolic process