Typical Testing Data/Standard Curve (for reference only)
Mouse Amyloid Beta Precursor Protein Binding Protein B3 (APBB3) ELISA Kit | APBB3 elisa kit
Mouse Amyloid Beta Precursor Protein Binding Protein B3 (APBB3) ELISA Kit
Gene Names
Apbb3; TR2S; Rirl2; Fe65l2
Reactivity
Mouse
Synonyms
Amyloid Beta Precursor Protein Binding Protein B3 (APBB3); Mouse Amyloid Beta Precursor Protein Binding Protein B3 (APBB3) ELISA Kit; APB-B3; SRA; FE65L2; FE65-L2; Fe65L2 isoform 484; Alzheimer's beta-amyloid interaction protein; Amyloid beta (A4) protein-binding; family B; member 3; isoform CRA_g; APBB3 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of APBB3. No significant cross-reactivity or interference between APBB3 and analogues was observed.
Sequence Length
486
Samples
Tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.625-40ng/mL
Sensitivity
<0.255ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level APBB3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level APBB3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for APBB3 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of APBB3 in mouse tissue homogenates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APBB3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to APBB3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APBB3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of APBB3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APBB3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to APBB3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APBB3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of APBB3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52,638 Da
NCBI Official Full Name
amyloid-beta A4 protein-binding family B member 3 isoform 1
NCBI Official Synonym Full Names
amyloid beta (A4) precursor protein-binding, family B, member 3
NCBI Official Symbol
Apbb3
NCBI Official Synonym Symbols
TR2S; Rirl2; Fe65l2
NCBI Protein Information
amyloid-beta A4 precursor protein-binding family B member 3; amyloid beta A4 precursor protein-binding family B member 3
UniProt Protein Name
Amyloid-beta A4 precursor protein-binding family B member 3
Protein Family
UniProt Gene Name
Apbb3
Uniprot Description
May modulate the internalization of amyloid-beta precursor protein.
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Product Notes
The Mouse APBB3 apbb3 (Catalog #AAA456957) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA456957 ELISA Kit recognizes Mouse APBB3. It is sometimes possible for the material contained within the vial of "Amyloid Beta Precursor Protein Binding Protein B3 (APBB3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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