Rabbit anti-Human ABI1 Polyclonal Antibody | anti-ABI1 antibody

ABI1, NT (Abl Interactor 1, Abelson Interactor 1, Abi-1, Spectrin SH3 Domain-binding Protein 1, Eps8 SH3 Domain-binding Protein, Eps8-binding Protein, e3B1, Nap1-binding Protein, Nap1BP, Abl-binding Protein 4, AblBP4, SSH3BP1) (APC)

Cat. Num. AAA6460883

• Gene Names: ABI1; E3B1; ABI-1; ABLBP4; NAP1BP; SSH3BP; SSH3BP1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ABI1; Polyclonal Antibody; NT (Abl Interactor 1; Abelson Interactor 1; Abi-1; Spectrin SH3 Domain-binding Protein 1; Eps8 SH3 Domain-binding Protein; Eps8-binding Protein; e3B1; Nap1-binding Protein; Nap1BP; Abl-binding Protein 4; AblBP4; SSH3BP1) (APC); anti-ABI1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ABI1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ABI1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human ABI1 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ABI1 Antibody (N-term) in MCF-7, CEM, Jurkat cell line lysates (35ug/lane). ABI1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with ABI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of SK-Br-3 cells using ABI1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(Immunofluorescence analysis of ABI1 Antibody (N-term) with paraffin-embedded human brain tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.)

Product Categories/Family for anti-ABI1 antibody

Antibodies; Spectrin

References

1. Wang, C., et.al., Mol. Cancer Res. 5(10), 1031-1039 (2007). 2. Carabeo, R.A., et.al., Cell. Microbiol. 9(9), 2278-2288 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 61743942
• NCBI GeneID: 10006
• NCBI Accession #: NP_005461.2
• NCBI GenBank Nucleotide #: NM_005470.3
• UniProt Accession #: Q8IZP0; O15147; O76049; O95060; Q5T2R3; Q5T2R4; Q5T2R6; Q5T2R7; A9Z1Y6; B3KX62; B4DQ58; H7BXI6
• Molecular Weight: 53,912 Da
• NCBI Official Full Name: abl interactor 1 isoform a
• NCBI Official Synonym Full Names: abl-interactor 1
• NCBI Official Symbol: ABI1
• NCBI Official Synonym Symbols: E3B1; ABI-1; ABLBP4; NAP1BP; SSH3BP; SSH3BP1
• NCBI Protein Information: abl interactor 1; Abelson interactor 1; nap1 binding protein; abl-binding protein 4; interactor protein AblBP4; Abl-interactor protein 1 long; eps8 SH3 domain-binding protein; spectrin SH3 domain-binding protein 1
• UniProt Protein Name: Abl interactor 1
• Protein Family: Abl interactor
• UniProt Gene Name: ABI1
• UniProt Synonym Gene Names: SSH3BP1; Abi-1; AblBP4; Eps8-binding protein; Nap1BP
• UniProt Entry Name: ABI1_HUMAN

NCBI Description

This gene encodes a member of the Abelson-interactor family of adaptor proteins. These proteins facilitate signal transduction as components of several multiprotein complexes, and regulate actin polymerization and cytoskeletal remodeling through interactions with Abelson tyrosine kinases. The encoded protein plays a role in macropinocytosis as a component of the WAVE2 complex, and also forms a complex with EPS8 and SOS1 that mediates signal transduction from Ras to Rac. This gene may play a role in the progression of several malignancies including melanoma, colon cancer and breast cancer, and a t(10;11) chromosomal translocation involving this gene and the MLL gene has been associated with acute myeloid leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 14. [provided by RefSeq, Sep 2011]

Uniprot Description

Abi-1: an SH3-containing adapter protein that regulates c-Abl-mediated phosphorylation of Mena. Binds the SH3 domains of epidermal growth factor receptor kinase substrate (EPS8) and of spectrin. May play a role in fusion of macropinocytic vesicles. Associated with congenital acute monocytic leukemia.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: postsynaptic membrane; growth cone; cytoskeleton; endoplasmic reticulum; lamellipodium; postsynaptic density; intracellular; nucleus; cytosol; cell junction; filopodium

Molecular Function: protein binding; cytoskeletal protein binding; protein complex binding; protein tyrosine kinase activator activity

Biological Process: negative regulation of cell proliferation; somitogenesis; peptidyl-tyrosine phosphorylation; actin polymerization and/or depolymerization; innate immune response; cell motility; vascular endothelial growth factor receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway