Rabbit anti-Human COCH Polyclonal Antibody | anti-COCH antibody

COCH, ID (COCH, COCH5B2, Cochlin, COCH-5B2)

Cat. Num. AAA645907

• Gene Names: COCH; DFNA9; COCH5B2; COCH-5B2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: COCH; Polyclonal Antibody; ID (COCH; COCH5B2; Cochlin; COCH-5B2); Anti -COCH; anti-COCH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-COCH antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50; Flow Cytometry: 1:10-50
• Immunogen: COCH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 398-428 amino acids from the Central region of human COCH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of COCH (arrow) in 293 cell lysates (2 ug/lane) using MBS645907 either nontransfected (Lane 1) or transiently transfected (Lane 2) with the COCH gene.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle using MBS645907 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS645907 for Immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Hela cells (right histogram) using MBS645907 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-COCH antibody

The protein encoded by this gene is highly conserved in human, mouse, and chicken, showing 94% and 79% amino acid identity of human to mouse and chicken sequences, respectively. Hybridization to this gene was detected in spindle-shaped cells located along nerve fibers between the auditory ganglion and sensory epithelium. These cells accompany neurites at the habenula perforata, the opening through which neurites extend to innervate hair cells. This and the pattern of expression of this gene in chicken inner ear paralleled the histologic findings of acidophilic deposits, consistent with mucopolysaccharide ground substance, in temporal bones from DFNA9 (autosomal dominant nonsyndromic sensorineural deafness 9) patients. Mutations that cause DFNA9 have been reported in this gene. Alternative splicing results in multiple transcript variants encoding the same protein. Additional splice variants encoding distinct isoforms have been described but their biological validities have not been demonstrated. [provided by RefSeq].

Product Categories/Family for anti-COCH antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 205277471
• NCBI GeneID: 1690
• NCBI Accession #: NP_001128530.1
• NCBI GenBank Nucleotide #: NM_001135058.1
• UniProt Accession #: O43405; A8K9K9; D3DS84
• Molecular Weight: 59,483 Da
• NCBI Official Full Name: cochlin
• NCBI Official Synonym Full Names: cochlin
• NCBI Official Symbol: COCH
• NCBI Official Synonym Symbols: DFNA9; COCH5B2; COCH-5B2
• NCBI Protein Information: cochlin; coagulation factor C homolog, cochlin (Limulus polyphemus)
• UniProt Protein Name: Cochlin
• Protein Family: Cochlin
• UniProt Gene Name: COCH
• UniProt Synonym Gene Names: COCH5B2
• UniProt Entry Name: COCH_HUMAN

NCBI Description

The protein encoded by this gene is highly conserved in human, mouse, and chicken, showing 94% and 79% amino acid identity of human to mouse and chicken sequences, respectively. Hybridization to this gene was detected in spindle-shaped cells located along nerve fibers between the auditory ganglion and sensory epithelium. These cells accompany neurites at the habenula perforata, the opening through which neurites extend to innervate hair cells. This and the pattern of expression of this gene in chicken inner ear paralleled the histologic findings of acidophilic deposits, consistent with mucopolysaccharide ground substance, in temporal bones from DFNA9 (autosomal dominant nonsyndromic sensorineural deafness 9) patients. Mutations that cause DFNA9 have been reported in this gene. Alternative splicing results in multiple transcript variants encoding the same protein. Additional splice variants encoding distinct isoforms have been described but their biological validities have not been demonstrated. [provided by RefSeq, Oct 2008]

Uniprot Description

Function: Plays a role in the control of cell shape and motility in the trabecular meshwork. Ref.8

Subunit structure: Interacts with SLC44A2. Interacts with ANXA2. Ref.7 Ref.8

Subcellular location: Secreted › extracellular space › extracellular matrix Ref.6.

Tissue specificity: Expressed in inner ear structures; the cochlea and the vestibule.

Post-translational modification: N-glycosylated. Ref.6A 50 kDa form is created by proteolytic cleavage.

Involvement in disease: Deafness, autosomal dominant, 9 (DFNA9) [MIM:601369]: A form of non-syndromic hearing loss characterized by onset in the fourth or fifth decade of life and initially involves the high frequencies. Hearing loss is progressive and usually complete by the sixth decade. In addition to cochlear involvement, DFNA9 patients also exhibit a spectrum of vestibular dysfunctions. Penetrance of the vestibular symptoms is often incomplete, and some patients are minimally affected, whereas others suffer from severe balance disturbances and episodes of vertigo. Affected individuals have mucopolysaccharide depositions in the channels of the cochlear and vestibular nerves. These depositions apparently cause strangulation and degeneration of dendritic fibers.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10 Ref.11 Ref.12 Ref.13 Ref.15 Ref.16 Ref.17

Sequence similarities: Contains 1 LCCL domain.Contains 2 VWFA domains.