Rabbit anti-Human, Mouse Pkmyt1 Polyclonal Antibody | anti-PKMYT1 antibody

Pkmyt1, NT (Pkmyt1, Myt1, Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase)

Cat. Num. AAA645787

• Gene Names: PKMYT1; MYT1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Pkmyt1; Polyclonal Antibody; NT (Pkmyt1; Myt1; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; Myt1 kinase); Anti -Pkmyt1; anti-PKMYT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PKMYT1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: Mouse Pkmyt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-143 amino acids from the N-terminal region of Mouse Pkmyt1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Pkmyt1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Pkmyt1 antibody detected the Pkmyt1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of Pkmyt1 (arrow) using rabbit polyclonal Mouse Pkmyt1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Pkmyt1 gene.)

Western Blot (WB)

(Mouse Pkmyt1 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Pkmyt1 antibody detected the Pkmyt1 protein (arrow).)

Related Product Information for anti-PKMYT1 antibody

Pkmyt1 acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development (By similarity).

Product Categories/Family for anti-PKMYT1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 113197982
• NCBI GeneID: 9088
• UniProt Accession #: Q99640; O14731; Q7LE24; Q8TCM9; B3KUN8; B4DXD4; D3DUA4; F8W164; I3L1V2
• Molecular Weight: 54,521 Da
• NCBI Official Full Name: PKMYT1 protein
• NCBI Official Synonym Full Names: protein kinase, membrane associated tyrosine/threonine 1
• NCBI Official Symbol: PKMYT1
• NCBI Official Synonym Symbols: MYT1
• NCBI Protein Information: membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; myt1 kinase
• UniProt Protein Name: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• Protein Family: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• UniProt Gene Name: PKMYT1
• UniProt Synonym Gene Names: MYT1
• UniProt Entry Name: PMYT1_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

Function: Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development. Ref.2 Ref.10

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Negatively regulated by hyperphosphorylation during mitosis. The hyperphosphorylated form does not associate with CCNB1-CDC2 complexes. The PLK1 protein kinase may be required for mitotic phosphorylation. Ref.1

Subunit structure: Interacts with CDC2-CCNB1 complex. Can also interact with PIN1 when phosphorylated by CDC2-CCNB1. Ref.8 Ref.9

Subcellular location: Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein Ref.2.

Domain: The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.

Post-translational modification: Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity. Ref.9 Ref.11

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.Contains 1 protein kinase domain.

Sequence caution: The sequence CAD28540.1 differs from that shown. Reason: Chimeric cDNA.