Western Blot (WB)
(Mouse Pkmyt1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Pkmyt1 antibody detected the Pkmyt1 protein (arrow).)
Rabbit anti-Human, Mouse Pkmyt1 Polyclonal Antibody | anti-Pkmyt1 antibody
Pkmyt1, NT (Pkmyt1, Myt1, Membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase, Myt1 kinase) (PE)
Gene Names
Pkmyt1; Myt1; AW209059; 6230424P17
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Pkmyt1; Polyclonal Antibody; NT (Pkmyt1; Myt1; Membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase; Myt1 kinase) (PE); Myt1 kinase; anti-Pkmyt1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-Pkmyt1 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Mouse Pkmyt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-143 amino acids from the N-terminal region of Mouse Pkmyt1.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Mouse Pkmyt1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Pkmyt1 antibody detected the Pkmyt1 protein (arrow).)
Western Blot (WB)
(Mouse Pkmyt1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Pkmyt1 antibody detected the Pkmyt1 protein (arrow).)
Western Blot (WB)
(Western blot analysis of Pkmyt1 (arrow) using rabbit polyclonal Mouse Pkmyt1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Pkmyt1 gene.)
Western Blot (WB)
(Western blot analysis of Pkmyt1 (arrow) using rabbit polyclonal Mouse Pkmyt1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Pkmyt1 gene.)
Western Blot (WB)
(Mouse Pkmyt1 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Pkmyt1 antibody detected the Pkmyt1 protein (arrow).)
Western Blot (WB)
(Mouse Pkmyt1 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Pkmyt1 antibody detected the Pkmyt1 protein (arrow).)
Related Product Information for anti-Pkmyt1 antibody
Pkmyt1 acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development (By similarity).
Product Categories/Family for anti-Pkmyt1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,053 Da
NCBI Official Full Name
membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
NCBI Official Synonym Full Names
protein kinase, membrane associated tyrosine/threonine 1
NCBI Official Symbol
Pkmyt1
NCBI Official Synonym Symbols
Myt1; AW209059; 6230424P17
NCBI Protein Information
membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; MYT1 kinase; membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase
UniProt Protein Name
Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
UniProt Gene Name
Pkmyt1
UniProt Synonym Gene Names
Myt1
UniProt Entry Name
PMYT1_MOUSE
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Product Notes
The Pkmyt1 pkmyt1 (Catalog #AAA6334442) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Pkmyt1, NT (Pkmyt1, Myt1, Membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase, Myt1 kinase) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Pkmyt1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Pkmyt1 pkmyt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pkmyt1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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