Western Blot (WB)
(Western Blot analysis in Y79 cell line lysates (35ug/lane) using MBS645730. This demonstrates that MBS645730 detected the EPB49 protein (arrow).)
Rabbit anti-Human EPB49 Polyclonal Antibody | anti-DMTN antibody
EPB49, ID (EPB49, DMT, Dematin, Erythrocyte membrane protein band 4.9)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(Western Blot analysis in Y79 cell line lysates (35ug/lane) using MBS645730. This demonstrates that MBS645730 detected the EPB49 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in Y79 cell line lysates (35ug/lane) using MBS645730. This demonstrates that MBS645730 detected the EPB49 protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
Dematin, or EPB49, is an actin-bundling protein originally identified in the erythroid membrane skeleton. Its actin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase and is restored after dephosphorylation (Rana et al., 1993 [PubMed 8341682]).[supplied by OMIM, Apr 2009]
Uniprot Description
Function: Membrane-cytoskeleton-associated protein with F-actin-binding activity that induces F-actin bundles formation and stabilization. Its F-actin-bundling activity is reversibly regulated upon its phosphorylation by the cAMP-dependent protein kinase A (PKA). Binds to the erythrocyte membrane glucose transporter-1 SLC2A1/GLUT1, and hence stabilizes and attaches the spectrin-actin network to the erythrocytic plasma membrane. Plays a role in maintaining the functional integrity of PKA-activated erythrocyte shape and the membrane mechanical properties. Plays also a role as a modulator of actin dynamics in fibroblasts; acts as a negative regulator of the RhoA activation pathway. In platelets, functions as a regulator of internal calcium mobilization across the dense tubular system that affects platelet granule secretion pathways and aggregation. Also required for the formation of a diverse set of cell protrusions, such as filopodia and lamellipodia, necessary for platelet cell spreading, motility and migration. Acts as a tumor suppressor and inhibits malignant cell transformation. Ref.7 Ref.8 Ref.9 Ref.13 Ref.17 Ref.19
Subunit structure: Monomeric (isoform 2); under reducing conditions. Self-associates. Exists under oxidizing condition as a trimer of two isoforms 2 and isoform 1 linked by disulfide bonds
Probable. Found in a complex with DMTN, F-actin and spectrin. Found in a complex with ADD2, DMTN and SLC2A1. Interacts with F-actin, ITPKB, RASGRF2 and spectrin. Isoform 2 interacts with SLC2A1 (via C-terminus cytoplasmic region). Isoform 1 and isoform 2 interact (phosphorylated form) with plasmodium berghei 14-3-3 protein; the interaction occurs in a PKA-dependent manner. Ref.2 Ref.8 Ref.9 Ref.13 Ref.16 Ref.17 Ref.18 Ref.19
Subcellular location: Cytoplasm. Cytoplasm › cytosol. Cytoplasm › perinuclear region
By similarity. Cytoplasm › cytoskeleton. Cell membrane. Membrane
By similarity. Endomembrane system. Cell projection
By similarity. Note: Localized at the spectrin-actin junction of erythrocyte plasma membrane. Localized to intracellular membranes and the cytoskeletal network. Localized at intracellular membrane-bounded organelle compartment in platelets that likely represent the dense tubular network membrane. Detected at the cell membrane and at the parasitophorous vacuol in malaria-infected erythrocytes at late stages of plasmodium berghei or falciparum development. Ref.8 Ref.16 Ref.18
Tissue specificity: Expressed in platelets (at protein level). Expressed in heart, brain, lung, skeletal muscle, and kidney. Ref.18
Domain: Both the N-terminal core domain and the C-terminal headpiece domain are sufficient for binding to F-actin and necessary for actin bundling activity.
Post-translational modification: Phosphorylated. Phosphorylation at Ser-403 by PKA causes the C-terminal headpiece domain to associate with the N-terminal core domain, and leads to the inhibition of its actin bundling activity. Ref.2 Ref.17 Ref.19 Ref.21The N-terminus is blocked.
Sequence similarities: Belongs to the villin/gelsolin family.Contains 1 HP (headpiece) domain.
Research Articles on DMTN
Similar Products
Product Notes
The DMTN dmtn (Catalog #AAA645730) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EPB49, ID (EPB49, DMT, Dematin, Erythrocyte membrane protein band 4.9) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EPB49 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the DMTN dmtn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPB49, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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