Rabbit anti-Human EPB49 Polyclonal Antibody | anti-EPB49 antibody

EPB49, ID (EPB49, DMT, Dematin, Erythrocyte membrane protein band 4.9) (MaxLight 750)

Cat. Num. AAA6295895

• Gene Names: DMTN; DMT; EPB49
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: EPB49; Polyclonal Antibody; ID (EPB49; DMT; Dematin; Erythrocyte membrane protein band 4.9) (MaxLight 750); Erythrocyte membrane protein band 4.9; anti-EPB49 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EPB49.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
• Sequence Length: 383

• Applicable Applications for anti-EPB49 antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 249-277 in the central region of human EPB49. Species sequence homology: bovine, mouse
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Y79 cell line lysates (35ug/lane) usingMBS645730. This demonstrates that MBS645730 detected the EPB49 protein (arrow).)

Related Product Information for anti-EPB49 antibody

Dematin, or EPB49, is an actin-bundling protein originally identified in the erythroid membrane skeleton. Its actin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase and is restored after dephosphorylation (Rana et al., 1993 [PubMed 8341682]).

Product Categories/Family for anti-EPB49 antibody

Antibodies; Membrane Proteins

References

1. Vugmeyster, L., et al. J. Biomol. NMR 47(2):155-162(2010). 2. Chen, L., et al. Protein Sci. 18(3):629-636(2009). 3. Vugmeyster, L., et al. J. Biomol. NMR 43(1):39-50(2009). 4. Vermeulen, W., et al. J. Mol. Biol. 359(5):1277-1292(2006). 5. Frank, B.S., et al. J. Biol. Chem. 279(9):7909-7916(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 166706881
• NCBI GeneID: 2039
• NCBI Accession #: NP_001107609.1; NP_001107608.1
• NCBI GenBank Nucleotide #: NP_001107609.1
• UniProt Accession #: Q08495
• NCBI Official Full Name: dematin isoform 2
• NCBI Official Synonym Full Names: dematin actin binding protein
• NCBI Official Symbol: DMTN
• NCBI Official Synonym Symbols: DMT; EPB49
• NCBI Protein Information: dematin
• UniProt Protein Name: Dematin
• UniProt Gene Name: EPB49
• UniProt Synonym Gene Names: DMT
• UniProt Entry Name: DEMA_HUMAN

NCBI Description

The protein encoded by this gene is an actin binding and bundling protein that plays a structural role in erythrocytes, by stabilizing and attaching the spectrin/actin cytoskeleton to the erythrocyte membrane in a phosphorylation-dependent manner. This protein contains a core domain in the N-terminus, and a headpiece domain in the C-terminus that binds F-actin. When purified from erythrocytes, this protein exists as a trimer composed of two 48 kDa polypeptides and a 52 kDa polypeptide. The different subunits arise from alternative splicing in the 3' coding region, where the headpiece domain is located. Disruption of this gene has been correlated with the autosomal dominant Marie Unna hereditary hypotrichosis disease, while loss of heterozygosity of this gene is thought to play a role in prostate cancer progression. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]

Uniprot Description

dematin: a probable DNA binding protein, with a Myb-like DNA-binding and ELM2 domain.

Protein type: Tumor suppressor; Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8p21.1

Cellular Component: cortical cytoskeleton; perinuclear region of cytoplasm; cell projection membrane; cytoplasmic membrane-bound vesicle; plasma membrane; endomembrane system; actin filament; cytosol; actin cytoskeleton

Molecular Function: protein binding; protein self-association; spectrin binding; actin binding; receptor binding

Biological Process: regulation of cell shape; actin filament bundle formation; negative regulation of peptidyl-tyrosine phosphorylation; erythrocyte development; positive regulation of blood coagulation; actin filament capping; regulation of actin cytoskeleton organization and biogenesis; negative regulation of focal adhesion formation; negative regulation of peptidyl-serine phosphorylation; cytoskeleton organization and biogenesis; regulation of filopodium formation; protein complex assembly; actin cytoskeleton organization and biogenesis