Goat KCNMA1 Polyclonal Antibody | anti-KCNMA1 antibody
KCNMA1 (Calcium-activated Potassium Channel Subunit alpha-1, BK Channel, BKCA alpha, Calcium-activated Potassium Channel, Subfamily M Subunit alpha-1, K(VCA)alpha, KCa1.1, Maxi K Channel, MaxiK, Slo-alpha, Slo1, Slowpoke Homolog, Slo Homolog, hSlo, KCNMA,
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: ELISA: 1:2000
NCBI and Uniprot Product Information
NCBI Description
MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit, which is the product of this gene, and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Potassium channel activated by both membrane depolarization or increase in cytosolic Ca2+ that mediates export of K+. It is also activated by the concentration of cytosolic Mg2+. Its activation dampens the excitatory events that elevate the cytosolic Ca2+ concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca2+, caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).
Enzyme regulation: Ethanol and carbon monoxide-bound heme increase channel activation. Heme inhibits channel activation. Ref.22
Subunit structure: Homotetramer; which constitutes the calcium-activated potassium channel. Interacts with RAB11B
By similarity. Interacts with beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. Interacts with gamma subunits LRRC26, LRRC38, LRRC52 and LRRC55. Beta and gamma subunits are accessory, and modulate its activity. Ref.18 Ref.19 Ref.20 Ref.21 Ref.26 Ref.28 Ref.29
Subcellular location: Cell membrane; Multi-pass membrane protein Ref.24 Ref.25.
Tissue specificity: Widely expressed. Except in myocytes, it is almost ubiquitously expressed. Ref.13
Domain: The S0 segment is essential for the modulation by the accessory beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. Ref.15 Ref.17The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor. Ref.15 Ref.17The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium. Ref.15 Ref.17The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel. It includes binding sites for Ca2+ and Mg2+
By similarity. Ref.15 Ref.17The calcium bowl constitutes one of the Ca2+ sensors and probably acts as a Ca2+-binding site. There are however other Ca2+ sensors regions required for activation of the channel. Ref.15 Ref.17The heme-binding motif mediates inhibition of channel activation by heme. Carbon monoxide-bound heme leads to increased channel activation. Ref.15 Ref.17
Post-translational modification: Phosphorylated
Probable. Phosphorylation by kinases such as PKA and/or PKG. In smooth muscles, phosphorylation affects its activity.Palmitoylation by ZDHHC22 and ZDHHC23 within the intracellular linker between the S0 and S1 transmembrane domains regulates localization to the plasma membrane. Depalmitoylated by LYPLA1 and LYPLAL1, leading to retard exit from the trans-Golgi network. Ref.24 Ref.25
Involvement in disease: Generalized epilepsy and paroxysmal dyskinesia (GEPD) [MIM:609446]: Epilepsy is one of the most common and debilitating neurological disorders. Paroxysmal dyskinesias are neurological disorders characterized by sudden, unpredictable, disabling attacks of involuntary movement often requiring life-long treatment. The coexistence of epilepsy and paroxysmal dyskinesia in the same individual or family is an increasingly recognized phenomenon. Patients manifest absence seizures, generalized tonic-clonic seizures, paroxysmal nonkinesigenic dyskinesia, involuntary dystonic or choreiform movements. Onset is usually in childhood and patients may have seizures only, dyskinesia only, or both.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.30
Miscellaneous: The protein was initially thought to contain two functionally distinct parts: The core channel (from the N-terminus to the S9 segment) that mediates the channel activity, and the cytoplasmic tail (from the S9 segment to the C-terminus) that mediates the calcium sensing. The situation is however more complex, since the core channel also contains binding sites for Ca2+ and Mg2+.
Sequence similarities: Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa1.1/KCNMA1 sub-subfamily. [View classification]Contains 1 RCK N-terminal domain.
Sequence caution: The sequence AAA50216.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the N-terminal part.The sequence AAB65837.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAC50353.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAK91504.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAD06365.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Research Articles on KCNMA1
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Product Notes
The KCNMA1 kcnma1 (Catalog #AAA645601) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The KCNMA1 (Calcium-activated Potassium Channel Subunit alpha-1, BK Channel, BKCA alpha, Calcium-activated Potassium Channel, Subfamily M Subunit alpha-1, K(VCA)alpha, KCa1.1, Maxi K Channel, MaxiK, Slo-alpha, Slo1, Slowpoke Homolog, Slo Homolog, hSlo, KCNMA, reacts with Bovine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KCNMA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA. Dilution: ELISA: 1:2000. Researchers should empirically determine the suitability of the KCNMA1 kcnma1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KCNMA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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