Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed U937 cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by AlexaFluor 488 secondary antibody (4ug/ml))
Goat ITGAM/CD11B Polyclonal Antibody | anti-ITGAM antibody
Goat anti-ITGAM/CD11B Antibody
Expected from sequence similarity: Human
Expected from sequence similarity: Human
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U937 cells. Recommended concentration: 10 ug/ml.
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed U937 cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by AlexaFluor 488 secondary antibody (4ug/ml))
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed U937 cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by AlexaFluor 488 secondary antibody (4ug/ml))
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Uniprot Description
ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes.
Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: cell surface; extracellular space; plasma membrane
Molecular Function: glycoprotein binding; protein binding
Biological Process: extracellular matrix organization and biogenesis; leukocyte migration; toll-like receptor 4 signaling pathway
Disease: Systemic Lupus Erythematosus, Susceptibility To, 6
Research Articles on ITGAM
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Product Notes
The ITGAM itgam (Catalog #AAA420973) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ITGAM/CD11B Antibody reacts with Tested: Human Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's ITGAM/CD11B can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA, Immunofluorescence (IF). Peptide ELISA: antibody detection limit dilution 1:32000. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U937 cells. Recommended concentration: 10 ug/ml. Researchers should empirically determine the suitability of the ITGAM itgam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITGAM/CD11B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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