Mouse anti-Human SECISBP2 Polyclonal Antibody | anti-SECISBP2 antibody

SECISBP2 (Selenocysteine Insertion Sequence-binding Protein 2, SECIS-binding Protein 2, SBP2)

Cat. Num. AAA644789

• Gene Names: SECISBP2; SBP2
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SECISBP2; Polyclonal Antibody; SECISBP2 (Selenocysteine Insertion Sequence-binding Protein 2; SECIS-binding Protein 2; SBP2); Anti -SECISBP2 (Selenocysteine Insertion Sequence-binding Protein 2; anti-SECISBP2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SECISBP2.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MASEGPREPESEGIKLSADVKPFVPRFAGLNVAWLESSEACVFPSSAATYYPFVQEPPVTEQKIYTEDMAFGASTFPPQYLSSEITLHPYAYSPYTLDSTQNVYSVPGSQYLYNQPSCYRGFQTVKHRNENTCPLPQEMKALFKKKTYDEKKTYDQQKFDSERADGTISSEIKSARGSHHLSIYAENSLKSDGYHKRTDRKSRIIAKNVSTSKPEFEFTTLDFPELQGAENNMSEIQKQPKWGPVHSVSTDISLLREVVKPAAVLSKGEIVVKNNPNESVTANAATNSPSCTRELSWTPMGYVVRQTLSTELSAAPKNVTSMINLKTIASSADPKNVSIPSSEALSSDPSYNKEKHIIHPTQKSKASQGSDLEQNEASRKNKKKKEKSTSKYEVLTVQEPPRIEDAEEFPNLAVASERRDRIETPKFQSKQQPQDNFKNNVKKSQLPVQLDLGGMLTALEKKQHSQHAKQSSKPVVVSVGAVPVLSKECASGERGRRMSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVGPAFTSDDTQDGESGGDDQFPEQAELSGPEGMDELISTPSVEDKSEEPPGTELQRDTEASHLAPNHTTFPKIHSRRFRDYCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLKKLKCVIISPNCEKIQSKGGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQDQFHKMVELTVAARQAYKTMLENVQQELVGEPRPQAPPSLPTQGPSCPAEDGPPALKEKEEPHYIEIWKKHLEAYSGCTLELEESLEASTSQMMNLNL

• Applicable Applications for anti-SECISBP2 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human SECISBP2, aa1-854 (AAH36109.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of SECISBP2 expression in transfected 293T cell line by SECISBP2 polyclonal antibody. Lane 1: SECISBP2 transfected lysate (93.94kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to SECISBP2 on HeLa cell. [antibody concentration 10ug/ml].)

Product Categories/Family for anti-SECISBP2 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 83779010
• NCBI GeneID: 79048
• NCBI Accession #: NP_076982.3
• NCBI GenBank Nucleotide #: NM_024077.4
• UniProt Accession #: Q96T21; Q5HYY1; Q8IYC0; Q9H0A1
• Molecular Weight: 95,462 Da
• NCBI Official Full Name: selenocysteine insertion sequence-binding protein 2 isoform 1
• NCBI Official Synonym Full Names: SECIS binding protein 2
• NCBI Official Symbol: SECISBP2
• NCBI Official Synonym Symbols: SBP2
• NCBI Protein Information: selenocysteine insertion sequence-binding protein 2
• UniProt Protein Name: Selenocysteine insertion sequence-binding protein 2
• Protein Family: Selenocysteine insertion sequence-binding protein
• UniProt Gene Name: SECISBP2
• UniProt Synonym Gene Names: SBP2; SECIS-binding protein 2
• UniProt Entry Name: SEBP2_HUMAN

NCBI Description

The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. The gene described in this record encodes a nuclear protein that functions as a SECIS binding protein. Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

SECISBP2: Binds to the SECIS element in the 3'-UTR of some mRNAs encoding selenoproteins. Binding is stimulated by SELB. Defects in SECISBP2 are a cause of abnormal thyroid hormone metabolism (ATHYHM). This phenotype is associated with a reduction in type II iodothyronine deiodinase activity. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: nucleoplasm; mitochondrion; ribonucleoprotein complex

Molecular Function: ribonucleoprotein binding; mRNA 3'-UTR binding

Biological Process: selenocysteine incorporation

Disease: Thyroid Hormone Metabolism, Abnormal