Rabbit anti-Human BCL2L11 Polyclonal Antibody | anti-BCL2L11 antibody

BCL2L11, ID (Bcl-2-like Protein 11, Bcl2-L-11, Bcl2-interacting Mediator of Cell Death, BIM)

Cat. Num. AAA642866

• Gene Names: BCL2L11; BAM; BIM; BOD
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: BCL2L11; Polyclonal Antibody; ID (Bcl-2-like Protein 11; Bcl2-L-11; Bcl2-interacting Mediator of Cell Death; BIM); Anti -BCL2L11; anti-BCL2L11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human BCL2L11. Reacts with amino acids near the center of human BCL2L11. Epitope: Internal
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-BCL2L11 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.; Dilution: Western Blot: 2.5ug/ml; Immunohistochemistry (Paraffin): 5ug/ml. Validated for use on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with B1090-32G, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
• Immunogen: Synthetic peptide corresponding to human BCL2L11. Species Sequence Homology: mouse and rat
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(MBS642866 (5ug/ml) was used to stain formalin-fixed, paraffin-embedded human heart followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(MBS642866 (5ug/ml) was used to stain formalin-fixed, paraffin-embedded human spleen followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-BCL2L11 antibody

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13kD, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.

Product Categories/Family for anti-BCL2L11 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 323362967
• NCBI GeneID: 10018
• NCBI Accession #: NP_001191039.1
• NCBI GenBank Nucleotide #: NM_001204110.1
• UniProt Accession #: O43521; O43522; Q0MSE7; Q0MSE8; Q0MSE9; Q53R28; Q6JTU6; Q6T851; Q6TE14; Q6TE15; Q6TE16; A8K2W2
• Molecular Weight: 22,171 Da
• NCBI Official Full Name: bcl-2-like protein 11 isoform 15
• NCBI Official Synonym Full Names: BCL2-like 11 (apoptosis facilitator)
• NCBI Official Symbol: BCL2L11
• NCBI Official Synonym Symbols: BAM; BIM; BOD
• NCBI Protein Information: bcl-2-like protein 11; bcl-2 interacting protein Bim; bcl-2-related ovarian death agonist; bcl-2 interacting mediator of cell death
• UniProt Protein Name: Bcl-2-like protein 11
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2L11
• UniProt Synonym Gene Names: BIM; Bcl2-L-11
• UniProt Entry Name: B2L11_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013]

Uniprot Description

BIM: a pro-apoptotic member of the BCL-2 protein family. Interacts with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and act as an apoptotic activator. Its expression can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role in neuronal and lymphocyte apoptosis. Transgenic studies in the mouse suggested that this protein functions as an essential initiator of the apoptosis in thymocyte-negative selection. Nineteen alternatively spliced transcript variants of this gene have been reported.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: microtubule; mitochondrial outer membrane; extrinsic to membrane; endomembrane system; cytosol

Molecular Function: protein binding; microtubule binding

Biological Process: nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; cell-matrix adhesion; pigmentation during development; myeloid cell homeostasis; ear development; B cell apoptosis; positive regulation of caspase activity; cellular process regulating host cell cycle in response to virus; positive regulation of apoptosis by virus; mammary gland development; B cell homeostasis; positive regulation of neuron apoptosis; T cell homeostasis; kidney development; post-embryonic organ morphogenesis; spleen development; caspase activation; thymus development; in utero embryonic development; positive regulation of protein homooligomerization; male gonad development; positive regulation of cell cycle; lumen formation; regulation of organ growth; odontogenesis of dentine-containing teeth; DNA damage response, signal transduction resulting in induction of apoptosis; spermatogenesis; regulation of pigmentation during development; brain development