Rabbit MAPK8/9/10 Polyclonal Antibody | anti-MAPK8/9/10 antibody

MAPK8/9/10, Phosphorylated (T183/Y185) (JNK, JNK1, PRKM8, SAPK1, JNK-46, JNK1A2, SAPK1c/JNK2, SAPK, p54a, JNK2A, JNK2B, PRKM9, JNK-55, SAPK1a, JNK2BETA, p54aSAPK, JNK2ALPHA/JNK3, JNK3A, PRKM10, SAPK1b, p493F12, p54bSAPK) (HRP)

Cat. Num. AAA6425829

• Gene Names: MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: MAPK8/9/10; Polyclonal Antibody; Phosphorylated (T183/Y185) (JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c/JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA/JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK) (HRP); anti-MAPK8/9/10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes MAPK8/9/10, phosphorylated T183/Y185. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)
• Sequence Length: 427

• Applicable Applications for anti-MAPK8/9/10 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 1:100-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding T183/Y185 of human MAPK8/MAPK9/MAPK10.
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from C6 cells untreated or treated with anisomycin using Phospho-MAPK8/9/10-T183/Y185 Antibody.)

Product Categories/Family for anti-MAPK8/9/10 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 2507195
• NCBI GeneID: 5599
• UniProt Accession #: P45983/P45984/P53779; P45983
• Molecular Weight: 4654
• NCBI Official Full Name: Mitogen-activated protein kinase 8
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8
• NCBI Official Symbol: MAPK8
• NCBI Official Synonym Symbols: JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
• NCBI Protein Information: mitogen-activated protein kinase 8

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]