Rabbit Bad Polyclonal Antibody
Bad, Phosphorylated (Ser112) (Bcl2 Antagonist of Cell Death, Bcl-2-binding Component 6, Bcl-xL/Bcl-2-associated Death Promoter, Bbc6)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
Bad; Polyclonal Antibody; Phosphorylated (Ser112) (Bcl2 Antagonist of Cell Death; Bcl-2-binding Component 6; Bcl-xL/Bcl-2-associated Death Promoter; Bbc6); Anti -Bad; anti-Bad antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Bad when phosphorylated at Ser112. Species Crossreactivity: human and rat.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% thimerosal, 50% glycerol.
Applicable Applications for anti-Bad antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1-2ug/ml
Immunoprecipitation: 5-20ug/ml
Dilution: Western Blot: 1-2ug/ml
Immunoprecipitation: 5-20ug/ml
Immunogen
Synthetic phosphopeptide corresponding to Ser112 from mouse Bad.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Bad antibody
Bad is a member of the Bcl-2 family protein that selectively dimerize with Bcl-xL and Bcl-2 resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser 136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-xL and Bcl-2. Recently, Akt has also been shown to promote cell survival via its ability to phosphorylate Bad at Ser136.
Product Categories/Family for anti-Bad antibody
Similar Products
Product Notes
The Bad (Catalog #AAA642502) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bad, Phosphorylated (Ser112) (Bcl2 Antagonist of Cell Death, Bcl-2-binding Component 6, Bcl-xL/Bcl-2-associated Death Promoter, Bbc6) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Bad can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1-2ug/ml Immunoprecipitation: 5-20ug/ml. Researchers should empirically determine the suitability of the Bad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bad, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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