Rabbit KCNA1 Polyclonal Antibody | anti-KCNA1 antibody

KCNA1 (Potassium Voltage-gated Channel Subfamily A Member 1, EA1, MK1, AEMK, HBK1, HUK1, MBK1, RBK1, KV1.1) (HRP)

Cat. Num. AAA6423618

• Gene Names: KCNA1; EA1; MK1; AEMK; HBK1; HUK1; MBK1; RBK1; KV1.1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: KCNA1; Polyclonal Antibody; KCNA1 (Potassium Voltage-gated Channel Subfamily A Member 1; EA1; MK1; AEMK; HBK1; HUK1; MBK1; RBK1; KV1.1) (HRP); anti-KCNA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes KCNA1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-KCNA1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human KCNA1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of U251 cell line, using KCNA1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using KCNA1 antibody.)

Product Categories/Family for anti-KCNA1 antibody

Antibodies; Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 119395748
• NCBI GeneID: 3736
• NCBI Accession #: NP_000208.2
• NCBI GenBank Nucleotide #: NM_000217.2
• UniProt Accession #: Q09470; Q3MIQ9; A6NM83
• Molecular Weight: 56,466 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily A member 1
• NCBI Official Synonym Full Names: potassium voltage-gated channel, shaker-related subfamily, member 1 (episodic ataxia with myokymia)
• NCBI Official Symbol: KCNA1
• NCBI Official Synonym Symbols: EA1; MK1; AEMK; HBK1; HUK1; MBK1; RBK1; KV1.1
• NCBI Protein Information: potassium voltage-gated channel subfamily A member 1; voltage-gated K(+) channel HuKI; voltage-gated potassium channel HBK1; voltage-gated potassium channel subunit Kv1.1
• UniProt Protein Name: Potassium voltage-gated channel subfamily A member 1
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: KCNA1
• UniProt Entry Name: KCNA1_HUMAN

NCBI Description

This gene encodes a voltage-gated delayed potassium channel that is phylogenetically related to the Drosophila Shaker channel. The encoded protein has six putative transmembrane segments (S1-S6), and the loop between S5 and S6 forms the pore and contains the conserved selectivity filter motif (GYGD). The functional channel is a homotetramer. The N-terminus of the channel is associated with beta subunits that can modify the inactivation properties of the channel as well as affect expression levels. The C-terminus of the channel is complexed to a PDZ domain protein that is responsible for channel targeting. Mutations in this gene have been associated with myokymia with periodic ataxia (AEMK). [provided by RefSeq, Jul 2008]

Uniprot Description

Kv1.1: Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. Defects in KCNA1 are the cause of episodic ataxia type 1 (EA1); also known as paroxysmal or episodic ataxia with myokymia (EAM) or paroxysmal ataxia with neuromyotonia. EA1 is an autosomal dominant disorder characterized by brief episodes of ataxia and dysarthria. Neurological examination during and between the attacks demonstrates spontaneous, repetitive discharges in the distal musculature (myokymia) that arise from peripheral nerve. Nystagmus is absent. Defects in KCNA1 are the cause of myokymia isolated type 1 (MK1). Myokymia is a condition characterized by spontaneous involuntary contraction of muscle fiber groups that can be observed as vermiform movement of the overlying skin. Electromyography typically shows continuous motor unit activity with spontaneous oligo- and multiplet-discharges of high intraburst frequency (myokymic discharges). Isolated spontaneous muscle twitches occur in many persons and have no grave significance. Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.1/KCNA1 sub-subfamily.

Protein type: Channel, potassium; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.32

Cellular Component: voltage-gated potassium channel complex; cell surface; endoplasmic reticulum; integral to plasma membrane; cytoplasmic membrane-bound vesicle; dendrite; paranode region of axon; integral to membrane; perikaryon; cytosol; presynaptic membrane; cell soma; apical plasma membrane; plasma membrane; synapse; nerve terminal; cell junction

Molecular Function: protein binding; voltage-gated potassium channel activity; potassium channel activity; delayed rectifier potassium channel activity; potassium ion transmembrane transporter activity

Biological Process: detection of mechanical stimulus involved in sensory perception of pain; synaptic transmission; regulation of membrane potential; regulation of muscle contraction; neuromuscular process; generation of action potential; protein homooligomerization; potassium ion transport; detection of mechanical stimulus involved in sensory perception of touch

Disease: Episodic Ataxia, Type 1