Rabbit CISD2 Polyclonal Antibody | anti-CISD2 antibody

CISD2 (CDGSH Iron-sulfur Domain-containing Protein 2, ERIS, Wolfram Syndrome 2, WFS2, ZCD2, NAF-1, Miner1) (FITC)

Cat. Num. AAA6411715

• Gene Names: CISD2; ERIS; WFS2; ZCD2; NAF-1; Miner1
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: CISD2; Polyclonal Antibody; CISD2 (CDGSH Iron-sulfur Domain-containing Protein 2; ERIS; Wolfram Syndrome 2; WFS2; ZCD2; NAF-1; Miner1) (FITC); anti-CISD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes CISD2. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-CISD2 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human CISD2.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

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Product Categories/Family for anti-CISD2 antibody

Antibodies; Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 56605994
• NCBI GeneID: 493856
• NCBI Accession #: NP_001008389
• NCBI GenBank Nucleotide #: NM_001008388.4
• UniProt Accession #: Q8N5K1; Q7Z3D5
• Molecular Weight: Predicted: 15 kDa; ; Observed: 15 kDa
• NCBI Official Full Name: CDGSH iron-sulfur domain-containing protein 2
• NCBI Official Synonym Full Names: CDGSH iron sulfur domain 2
• NCBI Official Symbol: CISD2
• NCBI Official Synonym Symbols: ERIS; WFS2; ZCD2; NAF-1; Miner1
• NCBI Protein Information: CDGSH iron-sulfur domain-containing protein 2; mitoNEET-related 1 protein; zinc finger, CDGSH-type domain 2; nutrient-deprivation autophagy factor-1; endoplasmic reticulum intermembrane small protein
• UniProt Protein Name: CDGSH iron-sulfur domain-containing protein 2
• Protein Family: CDGSH iron-sulfur domain-containing protein
• UniProt Gene Name: CISD2
• UniProt Synonym Gene Names: CDGSH2; ERIS; ZCD2; Miner1; NAF-1
• UniProt Entry Name: CISD2_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger protein that localizes to the endoplasmic reticulum. The encoded protein binds an iron/sulfur cluster and may be involved in calcium homeostasis. Defects in this gene are a cause of Wolfram syndrome 2. [provided by RefSeq, Mar 2011]

Uniprot Description

CISD2: a single-pass membrane protein of the endoplasmic reticulum that regulates autophagy. Has also been reported to localize to the mitochondrion outer membrane. Antagonizes ATG6-mediated cellular autophagy. Participates in the interaction of BCL2 with BECN1 and is required for BCL2-mediated depression of endoplasmic reticulum Ca2+ stores during autophagy. Contributes to BIK-initiated autophagy, while it is not involved in BIK-dependent activation of caspases. Involved in life span control, probably via its function as regulator of autophagy. Directly interacts with BCL2; the interaction is disrupted by BIK interaction with BCL2. It has been reported to mainly localizes to the mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome 2 (WFS2), is a rare autosomal recessive disorder characterized by optic atrophy and diabetes mellitus. Other symptoms include the presence of profound upper gastrointestinal ulceration, significant bleeding tendency, defective platelet aggregation with collagen and various neurological symptoms. Initially thought to be a zinc-finger protein, it was later shown to bind 1 2Fe-2S cluster instead.

Protein type: Autophagy; Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; protein complex; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: 2 iron, 2 sulfur cluster binding; protein binding; protein homodimerization activity; metal ion binding

Biological Process: mitochondrion degradation; multicellular organismal aging; regulation of autophagy

Disease: Wolfram Syndrome 2