Western Blot (WB)
(Western Blot of human Spleen Lysate using.)
Mouse anti-Human, Mouse CD63 Monoclonal Antibody | anti-CD63 antibody
CD63 (gp55, granulophysin, Lysosomal-associated membrane protein 3 (LAMP-3), Mast cell antigen AD1, melanoma 1 antigen, Melanoma-associated antigen MLA1, Melanoma-associated antigen ME491, MLA1, NGA, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Te
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
CD63; Monoclonal Antibody; CD63 (gp55; granulophysin; Lysosomal-associated membrane protein 3 (LAMP-3); Mast cell antigen AD1; melanoma 1 antigen; Melanoma-associated antigen MLA1; Melanoma-associated antigen ME491; MLA1; NGA; Ocular melanoma-associated antigen; OMA81H; PTLGP40; Te; gp55; Tetraspanin-30; TSPAN30; anti-CD63 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
MX-49.129.5
Specificity
Recognizes human CD63. Species Crossreactivity: mouse
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Applicable Applications for anti-CD63 antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Full length CD63 of human origin. Cellular Localization: Cytoplasmic and cell surface
Conjugate
MaxLight405
Positive Control
SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot of human Spleen Lysate using.)
Western Blot (WB)
(Western Blot of human Spleen Lysate using.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human Melanoma stained with.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human Melanoma stained with.)
Product Categories/Family for anti-CD63 antibody
References
1. C. Vennegoor et al., Int. J. Cancer 35: 287-295, 1985. 2. AA Palmer et al., Pathology 17: 335-339, 1985. 3. EC Hagen et al., Histopathology 10: 689-700, 1986.
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Product Notes
The CD63 (Catalog #AAA6193734) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD63 (gp55, granulophysin, Lysosomal-associated membrane protein 3 (LAMP-3), Mast cell antigen AD1, melanoma 1 antigen, Melanoma-associated antigen MLA1, Melanoma-associated antigen ME491, MLA1, NGA, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Te reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD63 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CD63 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD63, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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