Rabbit AQP3 Polyclonal Antibody | anti-AQP3 antibody

AQP3 (Aquaglyceroporin-3) (Biotin)

Cat. Num. AAA6404162

• Gene Names: AQP3; GIL; AQP-3
• Reactivity: Human, Mouse, Rat
• Applications: Immunocytochemistry, Western Blot, Immunohistochemistry
• Purity: Purified by Immunoaffinity chromatography
• Synonyms: AQP3; Polyclonal Antibody; AQP3 (Aquaglyceroporin-3) (Biotin); anti-AQP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AQP3. Species Crossreactivity: mouse and rat AQP3. No crossreactivity with other proteins.
• Purity/Purification: Purified by Immunoaffinity chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.

• Applicable Applications for anti-AQP3 antibody: Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ICC: 0.5-1ug/ml; WB: 0.1-0.5ug/ml; Applications are based on unconjugated antibody.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human AQP3, different from the related rat and mouse sequences by one amino acid.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-AQP3 antibody

Antibodies; Aquaporin

References

1. Ishibashi, K., Sasaki, S., Fushimi, K., Uchida, S., Kuwahara, M., Saito, H., Furukawa, T., Nakajima, K., Yamaguchi, Y., Gojobori, T., Marumo, F.Molecular cloning and expression of a member of the aquaporin family with permeability to glycerol and urea in addition to water expressed at the basolateral membrane of kidney collecting duct cells.Proc. Nat. Acad. Sci. 91: 6269-6273, 1994. 2. Mulders, S. M., Olde Weghuis, D., van Boxtel, J. A. F., Geurts van Kessel, A., Echevarria, M., van Os, C. H., Deen, P. M. T.Localization of the human gene for aquaporin 3 (AQP3) to chromosome 9, region p21-p12, using fluorescent in situ hybridization.Cytogenet. Cell Genet. 72: 303-305, 1996. 3. Sougrat, R., Morand, M., Gondran, C., Barre, P., Gobin, R., Bonte, F., Dumas, M., Verbavatz, J.-M.Functional expression of AQP3 in human skin epidermis and reconstructed epidermis.J. Invest. Derm. 118: 678-685, 2002.

NCBI and Uniprot Product Information

• NCBI GI #: 4826645
• NCBI GeneID: 360
• NCBI Accession #: NP_004916.1
• NCBI GenBank Nucleotide #: NM_004925.4
• UniProt Accession #: Q92482; O00108; Q6FGT2; Q6FGW6; A8K843; B2RE16; D3DRL3
• Molecular Weight: 31,544 Da
• NCBI Official Full Name: aquaporin-3
• NCBI Official Synonym Full Names: aquaporin 3 (Gill blood group)
• NCBI Official Symbol: AQP3
• NCBI Official Synonym Symbols: GIL; AQP-3
• NCBI Protein Information: aquaporin-3; aquaglyceroporin-3; aquaporin 3 (GIL blood group)
• UniProt Protein Name: Aquaporin-3
• Protein Family: Aquaporin
• UniProt Gene Name: AQP3
• UniProt Synonym Gene Names: AQP-3
• UniProt Entry Name: AQP3_HUMAN

NCBI Description

This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011]

Uniprot Description

AQP3: Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. Belongs to the MIP/aquaporin (TC 1.A.8) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter, aquaporin family; Membrane protein, integral; Transporter; Channel, misc.

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: integral to plasma membrane; basolateral plasma membrane; cytoplasm; plasma membrane; integral to membrane; intercellular junction

Molecular Function: transporter activity; urea transmembrane transporter activity; water channel activity; glycerol channel activity

Biological Process: odontogenesis; positive regulation of immune system process; glycerol transport; cellular water homeostasis; response to retinoic acid; response to vitamin D; transport; water transport; renal water homeostasis; response to calcium ion; excretion; regulation of keratinocyte differentiation; transmembrane transport

Disease: Gil Blood Group