Rabbit anti-Mouse Retnlb Polyclonal Antibody | anti-Retnlb antibody
Retnlb (Resistin-like beta, Cysteine-rich Secreted ProteinA12-beta, Cysteine-rich Secreted Protein FIZZ2, RELMbeta, Fizz2, 9030012B21Rik) (HRP)
Reactivity
Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
Retnlb; Polyclonal Antibody; Retnlb (Resistin-like beta; Cysteine-rich Secreted ProteinA12-beta; Cysteine-rich Secreted Protein FIZZ2; RELMbeta; Fizz2; 9030012B21Rik) (HRP); anti-Retnlb antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Retnlb.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-Retnlb antibody
Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
IHC-P: This antibody stained 4% PFA, paraffin-embedded sections of murine cecum tissue (with positive goblet cells of a Trichuris muris infected mouse). The primary antibody was incubated at a concentration of 0.33ng/ml overnight at 4 degree C and the secondary antibody was a cyanine-2 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM citric cid was used.
ELISA: To detect Murine RELMbeta by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5-2ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RELMbeta as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant Murine RELMbeta.
WB: To detect Murine RELMbeta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml when used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMbeta is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Applications are based on unconjugated antibody.
ELISA: To detect Murine RELMbeta by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5-2ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RELMbeta as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant Murine RELMbeta.
WB: To detect Murine RELMbeta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml when used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMbeta is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Applications are based on unconjugated antibody.
Immunogen
Highly purified mouse Retnlb.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Product Categories/Family for anti-Retnlb antibody
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Product Notes
The Retnlb (Catalog #AAA6403955) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Retnlb (Resistin-like beta, Cysteine-rich Secreted ProteinA12-beta, Cysteine-rich Secreted Protein FIZZ2, RELMbeta, Fizz2, 9030012B21Rik) (HRP) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Retnlb can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB). IHC-P: This antibody stained 4% PFA, paraffin-embedded sections of murine cecum tissue (with positive goblet cells of a Trichuris muris infected mouse). The primary antibody was incubated at a concentration of 0.33ng/ml overnight at 4 degree C and the secondary antibody was a cyanine-2 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM citric cid was used. ELISA: To detect Murine RELMbeta by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5-2ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RELMbeta as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant Murine RELMbeta. WB: To detect Murine RELMbeta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml when used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMbeta is 1.5-3ng/lane, under either reducing or non-reducing conditions. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Retnlb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Retnlb, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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