Chymase Active Protein | CMA1 active protein

Chymase, Recombinant, Human (CMA1)

Cat. Num. AAA638250

• Gene Names: CMA1; CYH; MCT1; chymase
• Purity: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Chymase; Recombinant; Human (CMA1); CMA1 active protein

• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a liquid in 25mM HEPES, 1.0M NaCl, pH 7.5.

• Activity: Measured by its ability to cleave a fluorogenic peptide substrate, N-Ala-Ala-Pro-Phe-AMC. The specific activity, measured with 100 M substrate and 100 ng activated enzyme in 100 L of 20 mM Tris, 2.0 M KCl, 0.02 % Triton X-100, pH 9.0 at room temperature, is > 80 pmoles/min/ g.
• Endotoxin Level: < 1.0 EU per 1 g of the protein as determined by the LAL method.
• Preparation and Storage: Samples are stable for up to six months at -20 degree C in a manual defrost freezer after the date of receipt. After receiving, this protein can be aliquoted and stored at above temperature for up to six months without a significant loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for CMA1 active protein

Chymases are a group of chymotrypsin-like serine proteases secreted by mast cells (1). They are synthesized as inactive precursors containing a 2-residue propeptide, which needs to be removed by dipeptidyl peptidase I/cathepsin C for the enzymatic activity (2). Human chymase encoded by the CMA1 gene is known to be involved in hypertention and heart failure through its ability to convert angiotensin I (Ang I) to angiotensin II (Ang II), which plays a key role in the regulation of arterial pressure (3). In addition, it is also important in physiological and pathological conditions including inflammation, fibrosis and processing of cytokines (4). Therefore, designing a specific inhibitor for chymase activity has been a pharmacologic strategy to develop therapeutic agents.

Product Categories/Family for CMA1 active protein

Molecular Biology; MB-Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4502907
• NCBI GeneID: 1215
• NCBI Accession #: NP_001827.1
• NCBI GenBank Nucleotide #: NM_001836.3
• UniProt Accession #: P23946; Q16018; Q3SY36; Q9UDH5; B5BUM8
• Molecular Weight: The secreted, purified rhChymase has an N-terminal sequence of G20 EIIGGTE, which corresponds to the pro form. The 238 amino acid His-tagged protein predicts a molecular mass of 27kD and migrates with an apparent molecular mass of approximately 38kD in SD
• NCBI Official Full Name: chymase preproprotein
• NCBI Official Synonym Full Names: chymase 1, mast cell
• NCBI Official Symbol: CMA1
• NCBI Official Synonym Symbols: CYH; MCT1; chymase
• NCBI Protein Information: chymase; alpha-chymase; chymase, heart; chymase, mast cell; mast cell protease I; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I
• UniProt Protein Name: Chymase
• Protein Family: Chymase
• UniProt Gene Name: CMA1
• UniProt Synonym Gene Names: CYH; CYM
• UniProt Entry Name: CMA1_HUMAN

NCBI Description

This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11.2 in a cluster of genes encoding other proteases. [provided by RefSeq, Jul 2008]

Uniprot Description

CMA1: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: Protease; Secreted; Secreted, signal peptide; EC 3.4.21.39

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular matrix; extracellular space; extracellular region; intracellular

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity; peptide binding

Biological Process: extracellular matrix disassembly; interleukin-1 beta biosynthetic process; positive regulation of angiogenesis; extracellular matrix organization and biogenesis; cellular protein metabolic process; regulation of inflammatory response; midbrain development; protein processing; angiotensin maturation; proteolysis