Chaperonin 10 Recombinant Protein
Chaperonin 10, Recombinant, Human (cpn10, Heat Shock Protein 10, Hsp10)
Applications
Western Blot
Purity
Highly Purified90% pure as determined by SDS-PAGE and Western Blot analysis.
Synonyms
Chaperonin 10; Recombinant; Human (cpn10; Heat Shock Protein 10; Hsp10); Chaperonin 10 recombinant protein
Host
E Coli
Purity/Purification
Highly Purified
90% pure as determined by SDS-PAGE and Western Blot analysis.
90% pure as determined by SDS-PAGE and Western Blot analysis.
Form/Format
Supplied as a liquid in 20mM Tris, pH 8.0, 150mM sodium chloride.
Applicable Applications for Chaperonin 10 recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 100ng On a 15-well minigel system recommended in a colorimetric.
Dilution: Western Blot: 100ng On a 15-well minigel system recommended in a colorimetric.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for Chaperonin 10 recombinant protein
Chaperonin 10 (cpn10) is the ~10kD mammalian equivalent to GroES of E. coli. Chaperonins (1) play fundamental roles in the folding, assembly and translocation of other proteins (2,3). Chaperonin function in vivo requires two distinct family members., cpn60 and cpn10, both of which are also present in chloroplasts and mitochondria. The best studied cpn60 and cpn10, are the GroEL and GroES proteins of E. coli, respectively. Cpn10 and cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly processes (2,3). Chaperonin 10 has been located in human platelets but more importantly it may also be present in human maternal serum (4,5). It has been reported (5) that human cpn10 is identical with early pregnancy factor (EPF), which is involved in control over cell growth and development. This identification suggests that cpn10 may act like a hormone in stressful situations such as pregnancy. Recombinant Chaperonin 10 protein (human) is also known as Heat Shock Protein 10 (Hsp10) or Mitochondrial Hsp10. It is reported to be identical to Early Pregnancy Factor (EPF).
Product Categories/Family for Chaperonin 10 recombinant protein
NCBI and Uniprot Product Information
Uniprot Description
HSPE1: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Belongs to the GroES chaperonin family.
Protein type: Mitochondrial
Chromosomal Location of Human Ortholog: 2q33.1
Cellular Component: mitochondrion; membrane; mitochondrial matrix
Molecular Function: protein binding; chaperone binding; unfolded protein binding; ATP binding
Biological Process: caspase activation; osteoblast differentiation; protein folding; response to unfolded protein
Similar Products
Product Notes
The Chaperonin 10 (Catalog #AAA638116) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Chaperonin 10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 100ng On a 15-well minigel system recommended in a colorimetric. Researchers should empirically determine the suitability of the Chaperonin 10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chaperonin 10, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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