Rabbit Cpn10 Polyclonal Antibody | anti-HSPE1 antibody

Cpn10 Antibody

Cat. Num. AAA805328

• Gene Names: HSPE1; EPF; CPN10; GROES; HSP10
• Reactivity: Human, Mouse, Rat, Bovine, Canine, Guinea pig, Pig, Rabbit, Sheep, Xenopus
• Applications: Western Blot, Immunoprecipitation, ELISA, Immunofluorescence
• Synonyms: Cpn10; Polyclonal Antibody; Cpn10 Antibody; 10kDa Chaperonin; Chaperonin 10; Cpn 10; EPF; GROES; Heat Shock 10kD protein1; Hsp10; HSPE1; anti-HSPE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine, Canine, Guinea pig, Pig, Rabbit, Sheep, Xenopus
• Clonality: Polyclonal
• Form/Format: Protein A Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 102

• Applicable Applications for anti-HSPE1 antibody: Westen Blot (WB), Immunoprecipitation (IP), ELISA (EIA), Immunofluorescence (IF)
• Application Notes: 1:1000 (WB)
• Certificate of Analysis: 1 ug/mL of SPC-193 was sufficient for detection of Cpn10 in 10ug of Hela lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Conjugate: Unconjugated
• Storage Buffer: PBS pH 7.3, 0.02% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis.)

Western Blot (WB)

(Western blot analysis of Rat brain cell lysates showing detection of Cpn10 protein using Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Related Product Information for anti-HSPE1 antibody

Background Info: Detects ~ 10kDa

Scientific Background: Chaperonin 10, otherwise known as Cpn10, (groES in E.coli) make up a family of small heart shock proteins with an approximate molecular mass of 10kDa (Hsp10s). Cpn10 acts as a co-chaperone and interacts with the Hsp60 family to promote proper folding of polypeptides. Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly process (1). Cpn10 has been located in human platelets, but is also present in human maternal serum (2, 3). It has been reported that human Cpn10 is identical with early pregnancy factor, which is involved in control over cell growth and development. This identification suggest that Cpn10 may act like a hormone in stressful situations such as pregnancy (4).

Product Categories/Family for anti-HSPE1 antibody

Chaperones; Heat Shock; Organelle Markers

References

1. Velez-Granell C.S., et al. (1994) J of Cell Science. 107(3): 539-549. 2. Morton H., Hegh V., and Clunie G.J.A. (1974) Nature (London) 249: 459-460. 3. Cavanagh A.C., and Morton H. (1994) Eur. J. Biochem. 222: 551-560. 4. Minto M., et al. (1998) Molecular Cell Research. 1403 (2): 151-157.

NCBI and Uniprot Product Information

• NCBI GI #: 4504523
• NCBI GeneID: 3336
• NCBI Accession #: NP_002148.1
• NCBI GenBank Nucleotide #: NM_002157.2
• UniProt Accession #: P61604; O95421; Q04984; Q53X54; Q9UDH0
• Molecular Weight: 10,932 Da
• NCBI Official Full Name: 10 kDa heat shock protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock 10kDa protein 1
• NCBI Official Symbol: HSPE1
• NCBI Official Synonym Symbols: EPF; CPN10; GROES; HSP10
• NCBI Protein Information: 10 kDa heat shock protein, mitochondrial; chaperonin 10; 10 kDa chaperonin; early-pregnancy factor; heat shock 10kD protein 1 (chaperonin 10)
• UniProt Protein Name: 10 kDa heat shock protein, mitochondrial
• UniProt Gene Name: HSPE1
• UniProt Synonym Gene Names: Hsp10; CPN10; EPF
• UniProt Entry Name: CH10_HUMAN

NCBI Description

This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011]

Uniprot Description

HSPE1: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Belongs to the GroES chaperonin family.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: mitochondrion; membrane; mitochondrial matrix

Molecular Function: protein binding; chaperone binding; unfolded protein binding; ATP binding

Biological Process: caspase activation; osteoblast differentiation; protein folding; response to unfolded protein