Cysteine-rich, Angiogenic Inducer 61 Active Protein | CYR61 active protein

Cysteine-rich, Angiogenic Inducer 61 (CYR61) Recombinant, Human

Cat. Num. AAA637502

• Gene Names: CYR61; CCN1; GIG1; IGFBP10
• Purity: Highly Purified; 95% by SDS-PAGE and HPLC analyses. Endotoxin: 1ng/ug.
• Synonyms: Cysteine-rich; Angiogenic Inducer 61; Angiogenic Inducer 61 (CYR61) Recombinant; Human; CYR61 active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% by SDS-PAGE and HPLC analyses. Endotoxin: 1ng/ug.
• Form/Format: Supplied as a lyophilized powder from 10mM sodium citrate, 50mM sodium chloride, pH 3.0. Reconstitute with sterile ddH2O and a carrier protein to a concentration of 0.1-1mg/ml.

• Biological Activity: Determined by the dose dependent stimulation of the proliferation of murine 3T3 cells. The expected ED50 for this effect is 2.0-3.0ug/ml.
• Country of Origin: USA
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile ddH2O and a carrier protein. Aliquot and store at -20 degree C. Reconstituted product is stable for 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CYR61 active protein

CYR61 is a member of the CCN family of secreted cysteine rich regulatory proteins. CYR61 induces angiogenesis by stimulating the proliferation, migration, and adhesion of endothelial cells. Cell migration and adhesion are mediated through binding to specific cell surface integrins and to heparin sulfate proteoglycans. Increased expression of CYR61 is associated with several types of cancer, and correlates with the progression and estrogen independence of human breast cancers. Recombinant human CYR61 is a 39.5kD protein containing 357 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the Thrombospondin type-I (TSP type-1) domain, and a C-terminal cysteine knot-like domain (CTCK

Product Categories/Family for CYR61 active protein

Growth Factors; Cytokines; Growth Factors-Other

NCBI and Uniprot Product Information

• NCBI GI #: 119593604
• NCBI GeneID: 3491
• UniProt Accession #: O00622; O14934; O43775; Q9BZL7
• Molecular Weight: 42,027 Da
• NCBI Official Full Name: cysteine-rich, angiogenic inducer, 61, isoform CRA_a
• NCBI Official Synonym Full Names: cysteine-rich, angiogenic inducer, 61
• NCBI Official Symbol: CYR61
• NCBI Official Synonym Symbols: CCN1; GIG1; IGFBP10
• NCBI Protein Information: protein CYR61; IBP-10; IGFBP-10; CCN family member 1; IGF-binding protein 10; cysteine-rich, anigogenic inducer, 61; cysteine-rich heparin-binding protein 61; insulin-like growth factor-binding protein 10
• UniProt Protein Name: Protein CYR61
• Protein Family: Protein
• UniProt Gene Name: CYR61
• UniProt Synonym Gene Names: CCN1; GIG1; IGFBP10; IBP-10; IGF-binding protein 10; IGFBP-10
• UniProt Entry Name: CYR61_HUMAN

NCBI Description

The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]

Uniprot Description

CYR61: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. Belongs to the CCN family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; extracellular matrix binding

Biological Process: anatomical structure morphogenesis; extracellular matrix organization and biogenesis; positive regulation of caspase activity; chemotaxis; intussusceptive angiogenesis; osteoblast differentiation; positive regulation of osteoblast differentiation; cell proliferation; cell-cell signaling; positive regulation of protein kinase activity; positive regulation of osteoblast proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of cell growth; positive regulation of protein amino acid phosphorylation; cell adhesion; positive regulation of BMP signaling pathway; negative regulation of apoptosis; positive regulation of cell migration