Rabbit anti-Human CCL4 Polyclonal Antibody | anti-CCL4 antibody

CCL4 (LAG1, MIP1B, SCYA4, C-C Motif Chemokine 4, G-26 T-lymphocyte-secreted Protein, HC21, Lymphocyte Activation Gene 1 Protein, Macrophage Inflammatory Protein 1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible Cytokine A4, T-cell Activation Prote

Cat. Num. AAA6372629

• Gene Names: CCL4; ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CCL4; Polyclonal Antibody; CCL4 (LAG1; MIP1B; SCYA4; C-C Motif Chemokine 4; G-26 T-lymphocyte-secreted Protein; HC21; Lymphocyte Activation Gene 1 Protein; Macrophage Inflammatory Protein 1-beta; PAT 744; Protein H400; SIS-gamma; Small-inducible Cytokine A4; T-cell Activation Prote; anti-CCL4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CCL4.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CCL4 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human CCL4, aa1-92 (NP_002975.1).
• Immunogen Sequence: MKLCVTVLSLLMLVAAFCSLALSAPMGSDPPTACCFSYTARKLPRNFVVDYYETSSLCSQPAVVFQTKRSKQVCADPSESWVQEYVYDLELN
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of CCL4 expression in transfected 293T cell line by CCL4 polyclonal antibody. Lane 1: CCL4 transfected lysate (10.2kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-CCL4 antibody

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. This protein is similar to CCL4 which inhibits HIV entry by binding to the cellular receptor CCR5. The copy number of this gene varies among individuals; most individuals have 1-5 copies in the diploid genome, although rare individuals do not contain this gene at all. The human genome reference assembly contains two copies of this gene. This record represents the more centromeric gene. [provided by RefSeq].

Product Categories/Family for anti-CCL4 antibody

Antibodies; Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 4506845
• NCBI GeneID: 6351
• NCBI Accession #: NP_002975.1
• NCBI GenBank Nucleotide #: NM_002984.2
• UniProt Accession #: P13236; P22617; Q13704; Q3SXL8; Q6FGI8
• Molecular Weight: 10,212 Da
• NCBI Official Full Name: C-C motif chemokine 4
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 4
• NCBI Official Symbol: CCL4
• NCBI Official Synonym Symbols: ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
• NCBI Protein Information: C-C motif chemokine 4; G-26 T-lymphocyte-secreted protein; MIP-1-beta(1-69); PAT 744; SIS-gamma; T-cell activation protein 2; lymphocyte activation gene 1 protein; macrophage inflammatory protein 1-beta; secreted protein G-26; small inducible cytokine A4
• UniProt Protein Name: C-C motif chemokine 4
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL4
• UniProt Synonym Gene Names: LAG1; MIP1B; SCYA4; LAG-1; MIP-1-beta; ACT-2
• UniProt Entry Name: CCL4_HUMAN

NCBI Description

The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. The encoded protein is secreted and has chemokinetic and inflammatory functions. [provided by RefSeq, Dec 2012]

Uniprot Description

CCL4: Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; extracellular region

Molecular Function: identical protein binding; protein binding; CCR1 chemokine receptor binding; chemokine activity; cytokine activity; CCR5 chemokine receptor binding

Biological Process: cell-cell signaling; response to toxin; response to virus; establishment and/or maintenance of cell polarity; positive regulation of calcium-mediated signaling; immune response; positive regulation of calcium ion transport; inflammatory response; cell motility; signal transduction; cell adhesion