Endoplasmic Reticulum Protein 29 Recombinant Protein | ERP29 recombinant protein

Recombinant Endoplasmic Reticulum Protein 29 (ERP29)

Cat. Num. AAA2033094

• Gene Names: ERP29; ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Endoplasmic Reticulum Protein 29; Recombinant Endoplasmic Reticulum Protein 29 (ERP29); C12orf8; ERp28; ERp31; PDI-DB; PDIA9; Protein Disulfide Isomerase Family A; Member 9; ERP29 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: DTVTFYKVI PKSKFVLVKF DTQYPYGEKQ DEFKRLAENS ASSDDLLVAE VGISDYGDKL NMELSEKYKL DKESYPVFYL FRDGDFENPV PYTGAVKVGA IQRWLKGQGV YLGMPGCLPVYDALAGEFIR ASG
• Sequence Length: 261

• Applicable Applications for ERP29 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 18.6kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Asp42~Gly173
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 5803013
• NCBI GeneID: 10961
• NCBI Accession #: NP_006808.1
• NCBI GenBank Nucleotide #: NM_006817.3
• Molecular Weight: 5,477 Da
• NCBI Official Full Name: endoplasmic reticulum resident protein 29 isoform 1
• NCBI Official Synonym Full Names: endoplasmic reticulum protein 29
• NCBI Official Symbol: ERP29
• NCBI Official Synonym Symbols: ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
• NCBI Protein Information: endoplasmic reticulum resident protein 29
• UniProt Protein Name: Endoplasmic reticulum resident protein 29
• Protein Family: Endoplasmic reticulum resident protein
• UniProt Gene Name: ERP29
• UniProt Synonym Gene Names: C12orf8; ERP28; ERp29; ERp28; ERp31
• UniProt Entry Name: ERP29_HUMAN

NCBI Description

This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

ERP29: Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; melanosome; membrane; smooth endoplasmic reticulum; transport vesicle

Molecular Function: chaperone binding; protein binding; protein disulfide isomerase activity; protein homodimerization activity

Biological Process: activation of MAPK activity; intracellular protein transport; negative regulation of protein secretion; positive regulation of protein amino acid phosphorylation; protein folding; protein secretion; protein unfolding