Rabbit anti-Human SEMA6A Polyclonal Antibody | anti-SEMA6A antibody

SEMA6A, CT (Semaphorin-6A, Semaphorin VIA, Sema VIA, Semaphorin-6A-1, SEMA6A-1, KIAA1368, SEMAQ) (AP)

Cat. Num. AAA6498747

• Gene Names: SEMA6A; VIA; SEMA; HT018; SEMAQ; SEMA6A1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: SEMA6A; Polyclonal Antibody; CT (Semaphorin-6A; Semaphorin VIA; Sema VIA; Semaphorin-6A-1; SEMA6A-1; KIAA1368; SEMAQ) (AP); anti-SEMA6A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SEMA6A.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-SEMA6A antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human SEMA6A (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SEMA6A Antibody (C-term) Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in HL-60 cell line lysate. SEMA6A (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-SEMA6A Antibody (C-term) pre-incubated with (right lane) and without (left lane) blocking peptide in mouse cerebellum tissue lysate. SEMA6A (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with SEMA6A antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-SEMA6A antibody

Antibodies; Plexin; Semaphorin

References

1. Prislei, S., Mol. Cancer Ther. 7(1), 233-241 (2008). 2. Katoh, M., Int. J. Mol. Med. 20(3), 405-409 (2007). 3. Gautier, G., Am. J. Pathol. 168(2), 453-465 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 11991660
• NCBI GeneID: 57556
• NCBI Accession #: NP_065847
• NCBI GenBank Nucleotide #: NM_020796
• UniProt Accession #: Q9H2E6; Q9P2H9
• Molecular Weight: 116,336 Da
• NCBI Official Full Name: semaphorin-6A isoform 2
• NCBI Official Synonym Full Names: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
• NCBI Official Symbol: SEMA6A
• NCBI Official Synonym Symbols: VIA; SEMA; HT018; SEMAQ; SEMA6A1
• NCBI Protein Information: semaphorin-6A; SEMA6A-1; sema VIa; semaphorin 6A-1; semaphorin VIA; semaphorin-6A-1
• UniProt Protein Name: Semaphorin-6A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA6A
• UniProt Synonym Gene Names: KIAA1368; SEMAQ; Sema VIA; SEMA6A-1

Uniprot Description

Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system. Can act as repulsive axon guidance cue. Has repulsive action towards migrating granular neurons. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation ().